A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP ...

1994年2月11日 · A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expressio …

Osamu Shimomura painstakingly isolated GFP from hundreds of thousands of jellyfish, characterized the chromophore and elucidated the mechanism of Aequorean bioluminescence. Martin Chalfie expressed the protein in E. coli and C. elegans, and Roger Tsien developed a palette of fluorescent proteins that could be used in a myriad of applications.

GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.

These GFP-like proteins allow the monitoring in time and space of an ever-increasing num-ber of phenomena in living cells and organisms like gene expression, protein localization and dynamics, protein-protein interactions, cell division, chromosome replication and organiza-tion, intracellular transport pathways, organelle inheritance and biogene...

Green Fluorescent Protein (GFP) refers to a protein isolated from the jellyfish (Aequorea victoria) that can be used as a reporter to trace lineages and determine cell fate in mouse and other model organisms.

2008年10月8日 · The very aptly named green fluorescent protein — or GFP as it is almost universally known — is a barrel-shaped protein made up of 238 amino acids.

2010年2月3日 · Martin Chalfie demonstrated the use of GFP as a luminescent reporter gene. In one of his pioneering experiments, he made six individual cells fluoresce by expressing GFP in the transparent roundworm C. elegans. Roger Y. Tsien contributed to a better understanding of the mechanism of GFP fluorescence.

2005年12月20日 · Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP,...

2009年10月1日 · This demonstration of GFP as a tool to study proteins in vivo fundamentally altered the nature and scope of the issues that could be addressed by cell biologists.