In CD1c + DCs, transduction of a GFP-RAB15 lentivector resulted in low expression of GFP-RAB15 and a small but significant decrease in infection with H1N1- and VSVG-pseudotyped viruses. To obtain cells with higher levels of RAB15 ectopic expression, we transduced monocytic THP-1 cells with blue fluorescent protein (BFP)–RAB15 or either BFP ...

2022年7月23日 · In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor...

2005年9月29日 · In this study, we identified the novel protein Rab15 effector protein (REP15) as a binding partner for Rab15-GTP. REP15 is compartment specific, colocalizing with Rab15 and Rab11 on the endocytic recycling compartment but not with Rab15, Rab4, or early endosome antigen 1 on sorting endosomes.

GFP expression in CD34-derived CD141 + DCs that were first transduced with the indicated shRNA lentivectors after infection of total CD34-derived cells with GFP-encoding HIV-1(BaL) Vpx or HIV-1...

2017年7月21日 · This study reveals that a subset of DCs that express CD141 is constitutively resistant to infection by a variety of viruses owing to the action of endocytic guanosine triphosphatase (GTPase) RAB15 but is able to cross-prime CD8 + T cells after acquiring viral antigens from infected bystander CD1c + DCs.

2005年3月11日 · In this study, we identified the novel protein Rab15 effector protein (REP15) as a binding partner for Rab15-GTP. REP15 is compartment specific, colocalizing with Rab15 and Rab11 on the endocytic recycling compartment but not with Rab15, Rab4, or early endosome antigen 1 on sorting endosomes.

2022年7月23日 · In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor recycling from the endocytic recycling compartment but otherwise remains poorly characterized. Here, we report …

Immunofluorescence assays revealed that GFP-tagged human RAB15 localized to Weibel-Palade bodies (WPBs), an endothelial cell-specific organelle, in HUVECs. Mapping Gross (2021) mapped the RAB15 gene to chromosome 14q23.3 based on an alignment of the RAB15 sequence (GenBank BC040679) with the genomic sequence (GRCh38).

2025年2月8日 · Rab15 effector protein is a binding partner for Rab15-GTP. Predicted to enable GTP binding activity and GTPase activity. Involved in positive regulation of regulated secretory pathway. Located in cilium; endosome membrane; and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Feb 2025]

2024年12月25日 · RAB15 (RAB15, Member RAS Oncogene Family) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Sertoli-Sertoli Cell Junction Dynamics. Gene Ontology (GO) annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RAB10.