The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2] [3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. In EGFP, the direction of the transition dipole moment is 14° from the line connecting the centers of the aromatic rings. (1996).

Here we characterize the excitation and emission spectra of some of the commonly used variants of GFP and recommend appropriate filter sets for detection and quantitation using the FL600 microplate fluorescence reader.

The GFP chromophore can exist as anionic or neutral, which explains the spectroscopic observation of two coincident absorbance and excitation bands . The neutral protonated or A form absorbs in the ultraviolet (UV) at about 395 nm, whereas the …

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

2008年10月8日 · Today all the colours of the rainbow can be found in a range of GFP and GFP-like proteins. Apart from the obvious biomedical implications, GFP sensors have also been developed that can...

GFP has major and minor excitation peaks at wavelengths of 395 and 475 nm, respectively. Several modifications have been made from the original GFP, most notably the reduction of the dual excitation peaks of 395 and 475 nm down to one excitation peak of 488 nm, which is in the visible blue-light range

A wide variety of amino acid substitutions to the original configuration have been successful in fine-tuning the fluorescence of native GFP to provide a broad range of derivative fluorophores that emit colors traversing from the blue to the yellow regions of the visible spectrum (see Figure 2).

2023年9月11日 · Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to …