Bacterial GFP strand 11 plasmid is a component of the Fold-N-Glow™ Protein Solubility Assay and contains a small (16 aa) GFP fragment for the fusion of the peptide or protein of interest via a flexible linker.

2021年9月16日 · We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in Corynactis californica, a bright red colonial anthozoan similar to sea anemones and...

2015年12月16日 · Live-cell functional uptake is detected when a CPP-protein fused to S11-peptide penetrates the cell membrane to complement cytosolic GFP1-10 protein, forming a functional GFP fluorophore.

GFP S11 is the small 16 amino acid strand 11 peptide of GFP that is detected by the GFP 1-10 by complementation to form fluorescent 11-stranded GFP. MBD2 is known to translocate to nuclei (Fig 2A). GFP 1-10 complements the GFP S11 tag, and the resulting GFP fluorescence is translocated to the nuclei (Fig 2B).

Split green fluorescent proteins (GFPs) are useful for making semisynthetic GFPs1-3containing unnatural amino acids with novel properties, for imaging and bioconjugation, and for fundamental studies of β-strand assembly and stability.

S11-tagged proteins, GFP fluorescence is easily detectable within 15 minutes after induction of the GFP 1-10 detector strand in E. coli cells. Transfer of these bacterial codon sequences to mammalian expression vectors gives detectable

engineered split GFP S11 detector fragment for tagging proteins with a C-terminal strand 11 of GFP “GFP S11”. These GFP S11-tagged proteins can be detected using the so-called GFP 1-10 “detector” fragment, a highly

2024年7月10日 · Cytosolic delivery of Lipofectamine-complexed IpaH9.8-K27-D25-s11 resulted in a 46% reduction of GFP-KRAS levels, whereas control proteins lacking E3, binding ability, or ApP could not deplete...

2023年12月27日 · In brief, the ccGFP E6 strands 1–10 (ccGFP 1–10 E6) and strand 11 (S11) fragments were engineered based on sequence alignments of the split GFP construct (Cabantous et al., 2005). The S11 was evolved to minimize aggregation when fused to E. coli hexulose phosphate synthase and the resulting variant named S11 v1 appeared to not perturb the ...

ipGFP1–9 with S10–11 peptide showed 49-times higher fluorescence recovery than refolded split GFP1–9. Molar ratios, pH and ionic strength were probed for reconstitution-based applications of split GFP system, even in vitro.