GFP (S65T) is a basic (constitutively fluorescent) green fluorescent protein published in 1995, derived from Aequorea victoria.

The S65T mutation rearranges the hydrogen bond pattern of Glu222, suppressing its negative charge, in turn allowing the chromophore to become ionized. 3 In PA-GFP, the exchange of His for Thr203 reduces hydrogen bonding opportunities to the chromophore hydroxyl group so that it preferentially remains neutral, with Glu222 carrying a negative charge.

Emerald包含F64L和S65T突变，另外还有四个点突变从而改进了折叠、37℃时的突变率以及亮度。 虽然Emerald比EGFP更有效，但含有快速光漂白成分，可能在某些环境下其定量成像会受到影响。

Plasmid pFA6a-GFP (S65T)-kanMX6 from Dr. John Pringle's lab contains the insert KanR and is published in Yeast. 1998 Jul;14 (10):943-51. This plasmid is available through Addgene.

Green fluorescent protein (GFP) has emerged as a powerful new tool in a variety of organisms. An engineered sGFP(S65T) sequence containing optimized codons of highly expressed eukaryotic proteins has provided up to 100-fold brighter fluorescence signals than the original jellyfish GFP sequence in pl …

Plasmid pRS402-GPD-GFP (S65T) from Dr. David Bartel's lab contains the insert EGFP and is published in Nature. 2012 Jun 20;486 (7403):368-74. doi: 10.1038/nature11211. This plasmid is available through Addgene.

1997年4月1日 · GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm. GFP-S65T was brightly fluorescent in solution (lambda ex 492 nm, lambda em 509 nm) with a lifetime of 2.9 ns and a rotational correlation time (tc) of 20 ns.

Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively. Excitation of either band leads to green emission.

提供 pfa6a-gfp(s65t)-trp1 载体/质粒图谱，全长序列，抗性，大小和元件信息。

1999年4月10日 · Recently, it has been found that the fluorescence spectra of most mutants of GFP respond rapidly and reversibly to pH variations, making them useful as probes of intracellular pH. To explore the structural basis for the titration behavior of the popular GFP S65T variant, we determined high-resolution crystal structures at pH 8.0 and 4.6.