The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2][3] The label GFP traditionally refers to the protein …

2020年9月4日 · We thus aimed at optimizing a protocol for improving the GFP signal in unfixed, snap-frozen tissue sections. To do so, we have compared several post-sectioning fixation …

GFP被广泛应用于生物学领域的研究，科学家们利用GFP实现多种功能，包括：标记基因以阐明其表达或定位特征，充当生物传感器或细胞标记，研究蛋白质之间的相互作用，可视化启动子的 …

We report that GFP is a reliable reporter of gene expression in individual eukaryotic cells when fluorescence is measured by flow cytometry. Two pieces of evidence that support this …

Despite its monolithic appearance, GFP can be expressed as two separate segments that nevertheless combine within cells to yield a functional fluorescent protein. 5,6 This discovery …

Green fluorescent protein (GFP) is an ideal reporter in in vivo studies. Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is …

2014年1月1日 · Green fluorescent protein (GFP) is an ideal reporter in in vivo studies. Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; …

SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1).

Green fluorescent protein (GFP) is autofluorescent. This property has made GFP useful in monitoring in vivo activities such as gene expression and protein localization. We find that …

1998年1月1日 · Spectral and physical properties of GFP affect the accuracy and usefulness of any quantitative measurement. Many of these properties, such as extinction coefficient, …