The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2] [3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

2021年11月1日 · Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsis and tobacco) and two...

First, GFP/S65T is primarily excited in the blue region of the visible spectrum, rather than in the UV as with wild-type and is therefore more useful for imaging applications. Second, several groups already had crystals of wild-type GFP and we felt that a comparison of wild-type versus mutant structures would immediately be useful.

2001年12月10日 · Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP 398)...

pGFPuv encodes a variant of Aequorea victoria green fluorescent protein (GFP) that has been optimized for brighter fluorescence when excited by standard UV light. The GFPuv coding sequence is flanked by separate MCSes at the 5' and 3' …

2025年3月7日 · To mimic the phenotype observed in these autoimmune photosensitive diseases, we generated a green fluorescent protein (GFP)–ZBP1–FLAG–tagged KC cell line of the long isoform [ZBP1 overexpressing (OE)] in N/TERTs and compared it with an N/TERT line expressing GFP alone (Fig. 7D and fig. S7, C and D). ZBP1 was primarily expressed in the ...

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page are copyrighted material.

We investigate the utility of 193 nm ultraviolet photodissociation (UVPD) in comparison to collision induced dissociation (CID), higher energy CID (HCD), and electron transfer dissociation (ETD) for top down fragmentation of highly homologous green fluorescent proteins (GFP) in the gas phase.

2023年9月26日 · 其实，紫外光可以激发野生型gfp，但不适用于增强型的绿色荧光蛋白egfp。今天，上海析浦科学仪器将给大家介绍常用的gfp变体的激发和发射光谱，帮助大家更好地选择合适的荧光蛋白激发光源。 绿色荧光蛋白gfp各种变体的激发光和发射光波长

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP (398)) into one absorbing at 483 nm (GFP (483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222.