The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2] [3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

2021年11月1日 · We observed bright fluorescence under UV light in all of the four plant species without any effects on plant growth or development. eYGFPuv was shown to be effective for imaging transient...

In PA-GFP, photoswitching is accomplished by brief exposure to UV light, during which Glu222 decarboxylates to become a neutral species, thus allowing the chromophore to become ionized. 23 Mechanism of fluorescence emission and excited state proton transfer

Bio-Rad offers two portable sources of UV light for detecting and visualizing green fluorescent protein (GFP), which fluoresces brilliant green when expressed in cells or cell extracts. This pocket-size disposable lamp has the power needed to illuminate your experiments.

2001年12月10日 · Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP 398)...

pGFPuv encodes a variant of Aequorea victoria green fluorescent protein (GFP) that has been optimized for brighter fluorescence when excited by standard UV light. The GFPuv coding sequence is flanked by separate MCSes at the 5' and 3' …

GFP variant optimized for excitation by UV light. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Sticky ends from different BanII sites may not be compatible. Sticky …

Wild-type GFP is a 27-kDa monomer consisting of 238 amino acids that has the unique characteristic of emitting green light when excited with UV (360–400 nm) or blue (440–480 nm) light. GFP is the only well-characte- r ized example of a protein that displays strong, visible fluorescence without any additional substrates or co-factors (14).

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process th …

2022年9月1日 · Can GFP be seen without UV light? It turns out that GFP is amazingly useful in scientific research, because it allows us to look directly into the inner workings of cells. It is easy to find out where GFP is at any given time: you just have to shine ultraviolet light, and any GFP will glow bright green.