In molecular biology, a GGDEF domain is a protein domain which appears to be ubiquitous in bacteria and is often linked to a regulatory domain, such as a phosphorylation receiver or oxygen sensing domain.

2022年5月2日 · Here, we show that direct interaction between the inactive diguanylate cyclase (GGDEF) domain of PdeB and the FimV domain of the polar landmark protein HubP is crucial for full function of PdeB...

2020年10月2日 · Diguanylate-cyclase (DGC) or GGDEF domain: Originally named after a conserved residue pattern, and initially described as a domain of unknown function 1 (DUF1). This domain is widely present in bacteria, linked to a wide range of non-homologous domains in a variety of cell signaling proteins.

The GGDEF domain plays an important role in c-di-GMP metabolism. On the one hand, GGDEF domain widely exists in DGCs (diguanylate cyclases), catalyzing the synthesis of c-di-GMP.

Identification of Diguanylate Cyclases (the GGDEF Domain) Diguanylate cyclase activity, synthesis of the bacterial second messenger c‐di‐GMP from two molecules of GTP, is a property of the so‐called GGDEF domain , named after its most conserved sequence motif, Gly‐Gly‐Asp/Glu‐Glu‐Phe ( Galperin et al. , 2001; Jenal and Malone ...

一方面,GGDEF结构域广泛存在于二鸟苷酸环化酶 (diguanylate cyclases,DGCs)上,催化c-di-GMP的合成;另一方面,GGDEF结构域也可与EAL等结构域串联,参与c-di-GMP的分解。 该文系统介绍了c-di-GMP的代谢过程及其生理作用,重点论述了GGDEF结构域有效调控c-di-GMP代谢的结构基础和机制,并讨论了该领域研究的应用价值与未来方向。

2008年5月23日 · 环状二聚体 GMP (c-di-GMP) 是一种重要的生物膜调节剂，可通过变构激活胞外多糖生物合成酶。 变形菌基因组通常编码多个含有 GGDEF 结构域的双鸟苷酸环化酶，负责 c-di-GMP 的合成。 相比之下，在测序的葡萄球菌基因组中仅存在一种保守的 GGDEF 结构域蛋白 GdpS（用于来自葡萄球菌的 GGDEF 结构域蛋白）和具有高度修饰的 GGDEF 结构域的第二种蛋白 GdpP。 在这里，我们调查了 GdpS 在表皮葡萄球菌生物膜形成中的作用。 在静态和流动 …

This work aims at highlighting these peculiar properties of the GGDEF domain and to propose some useful guidelines to minimize the structural and kinetics bias due to a possible disruption of the GGDEF conformational constraints in engineered constructs.

2024年2月1日 · High c-di-GMP levels driven by diguanylate cyclases (DGCs) activity favor biofilm formation, while low levels were maintained by phosphodiesterases (PDE) encourage planktonic lifestyle. The activity of these enzymes can be modulated by stimuli-sensing domains such as Per-ARNT-Sim (PAS).

2025年3月22日 · Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger that regulates multiple bacterial behaviors. It is synthesized by diguanylate cyclase (DGC) with the GGDEF domain, and degraded by phosphodiesterase (PDE) with the EAL or HD-GYP domain. The GepA (VP0117) protein in Vibrio parahaemolyticus contains both GGDEF and EAL domains, but its role remains unknown. This study found that ...