2014年5月1日 · The thick peptidoglycan layers of Gram-positive bacteria are connected to polyanionic glycopolymers called wall teichoic acids (WTA). Pathogens such as Staphylococcus aureus, Listeria monocytogenes, or Enterococcus faecalis produce WTA with diverse, usually strain-specific structure.

2025年3月1日 · WTA consist of polymerized ribitol-phosphate backbone that is modified with N-acetylglucosamine (GlcNAc) moieties in different configurations by the glycosyltransferases TarS (β−1,4-GlcNAc), TarM (α−1,4-GlcNAc) and TarP (β−1,3-GlcNAc).

2022年12月1日 · UDP-GlcNAc, which is required for peptidoglycan and wall teichoic acid (WTA) biosynthesis, is synthesized from Fructose-6-phosphate in a four-step reaction catalyzed by GlmS, GlmM, GlmU and GlmR. MurA, an UDP-Glc N Ac-1-carboxyvinyltransferase catalyzing the first step of peptidoglycan biosynthesis, and MurB convert UDP-Glc N Ac to UDP-Mur N Ac.

2024年11月4日 · Recent studies have shown that galactosylation of the cell wall polymer wall teichoic acid (WTA) is essential for InlB anchoring on the cell surface of L. monocytogenes serotype 4b strains. Galactosylation of WTA is exerted by the coordinated action of several glycosyltransferases, including GalU, GalE, GtcA, GttA, and GttB.

There are two types of teichoic acids: wall teichoic acid (WTA), which is attached to peptidoglycan by a disaccharide linkage unit, and lipoteichoic acid (LTA), which is attached to the plasma membrane by the Glc-DAG anchor.

WTA and complex cell wall polysaccharides can be glycosylated intracellularly and extracellularly. GT-C-fold glycosyltransferases can be predicted by structural homology analysis. Secondary cell wall polymers fulfil diverse and important functions within the cell wall of Gram-positive bacteria.

开发预防性或治疗性抗 MRSA 疫苗是治疗 金黄色葡萄球菌 感染和克服耐药危机的潜在方法。 来自金黄色葡萄球菌的β -1,4-GlcNAc糖基化壁磷壁酸（WTA）是一种与β-内酰胺耐药密切相关的新型抗原。 在本研究中，结构定义的 β通过离子液体支持的寡糖合成（ILSOS）策略，以高效、无色谱的方法合成了链长和 GlcNAc 修饰数量不同的 -1,4-GlcNAc 修饰的 WTA。 然后将获得的 WTA 与破伤风毒素 (TT) 结合作为候选疫苗，并在小鼠模型中进一步评估以确定结构-免疫原性关系。

2017年10月27日 · We unveiled more than 10 different types of WTA polymers that vary in their linkage and repeating units. Disparity in GlcNAc to ribitol connectivity, as well as variable O-acetylation and glycosylation of GlcNAc contribute to the structural diversity of WTAs. Notably, SPR analysis indicated that constitution of WTA determines the recognition by ...

2024年8月14日 · Using a tailored growth medium and purification protocols, we isolated 13 C-isotope labelled Glc 2-DAG from bacteria, which can then be used for high-resolution NMR studies. Using solution-state and solid-state NMR, we show an in-depth molecular characterization of Glc 2-DAG, including in native-like membranes.

Recent studies have shown that galactosylation of the cell wall polymer wall teichoic acid (WTA) is essential for InlB anchoring on the cell surface of L. monocytogenes serotype 4b strains. Galactosylation of WTA is exerted by the coordinated action of several glycosyltrans-ferases, including GalU, GalE, GtcA, GttA, and GttB.