2024年11月4日 · We found that glutamine anaplerosis into the tricarboxylic acid (TCA) cycle decreases during MuSC differentiation and coincides with decreased expression of the mitochondrial glutamate deaminase GLUD1. Deletion of Glud1 in proliferating MuSCs resulted in precocious differentiation and fusion, combined with loss of self-renewal in vitro and in vivo.

谷氨酰胺通过氨基酸转运蛋白 ASCT2/SLC1A5 进入细胞，并在线粒体中通过被谷氨酰胺酶 (GLS) 催化的脱氨反应转化为谷氨酸。 谷氨酸被谷氨酸脱氢酶 (GDH) 或丙氨酸或天冬氨酸转氨酶 (TAs) 转化为 TCA 循环中间物 α-酮戊二酸 (α-KG)。 此反应中，TAs 还会生成相应氨基酸。 α-KG 是一种关键的代谢物，可在 ATP 产生以及补充 TCA 循环中间物的过程（一种称为回补的过程）中发挥作用。 在缺氧或线粒体功能障碍期间，α-KG 在 IDH2 催化的还原性羧化反应中被转换为柠檬酸 …

2018年2月7日 · Mechanistically, the direct interaction between SIRT5 and glutamate dehydrogenase 1 (GLUD1) causes deglutarylation and functional activation of GLUD1, a critical regulator of cellular...

2023年4月3日 · During anaplerosis, mitochondrial glutamate dehydrogenase 1 (GLUD1) plays a key role by catalyzing the conversion of glutamate to alpha-ketoglutarate (α-KG) and...

2022年11月1日 · GLUD1, a key mitochondrial enzyme in glutaminolysis, is transactivated by the direct binding of leucine and converts glutamate to α-KG to enrich the TCA metabolite pool for rapid growth and proliferation [49]. It is also important for redox homeostasis in cancer cells by controlling the intracellular levels of its product α-KG and the ...

2015年2月9日 · Here we report that the mitochondrial enzyme glutamate dehydrogenase 1 (GDH1) is commonly upregulated in human cancers. GDH1 is important for redox homeostasis in cancer cells by controlling the intracellular levels of its product alpha-ketoglutarate and subsequent metabolite fumarate.

2024年10月1日 · Here, we discovered that deacetylation of glutamate dehydrogenase 1 (GLUD1), one of the key enzymes in glutamine catabolism, maintains the survival of lung adenocarcinoma (LUAD) cells under glucose starvation by inhibiting autophagic cell death.

We discovered that GLUD1 showed a high protein expression in lung adenocarcinoma cells or tissues. We elucidated that STIP1 homology and U-box-containing protein 1 (STUB1) was the key E3 ligase responsible for ubiquitin-mediated proteasomal degradation of GLUD1.

2023年10月4日 · 我们发现，在 MuSC 分化过程中，TCA 循环中的谷氨酰胺回补减少，并且与线粒体谷氨酸脱氨酶 GLUD1 表达的减少相一致。增殖的 MuSC 中 Glud1 的基因缺失导致早熟分化和不平衡融合，以及体外和体内自我更新的丧失。

2023年10月5日 · We found that glutamine anaplerosis into the TCA cycle decreases during MuSC differentiation and coincides with decreased expression of the mitochondrial glutamate deaminase GLUD1. Genetic deletion of Glud1 in proliferating MuSCs resulted in precocious differentiation and imbalanced fusion combined with loss of self-renewal in vitro and in vivo.