
Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA …
2010年3月3日 · Gp15 and gp16 actively translocate the T7 genome into an infected cell. The majority of the T7 genome is actively transported into the cell, at rates characteristic of the …
Reprogramming Bacteriophage Host Range through Structure ... - Cell …
2019年10月29日 · We identify Gp15 as the PSA RBP and construct a synthetic phage library featuring sequence-randomized RBPs, from which host range mutants are isolated and …
Stable expression of Cryptosporidium parvum glycoprotein …
One of these, Cpgp40/15, is expressed as a precursor protein that is cleaved by a parasite-derived furin-like protease activity into gp15, a glycophosphatidyl inositol anchored surface …
The Molecular Architecture of the Bacteriophage T4 Neck
2013年5月27日 · Here we report the crystal structure of the gp15 hexamer, describe its interactions in T4 virions that have either an extended tail or a contracted tail, and discuss its …
Molecular Cloning and Expression of a Gene Encoding …
We have cloned and sequenced a gene designated Cpgp40/15 that encodes gp40 as well as gp15, an antigenically distinct, surface glycoprotein also implicated in C. parvum -host cell …
Gp15 and gp16 cooperate in translocating bacteriophage T7 …
2010年3月15日 · We suggest that both gp15 and gp16 cooperate to form a molecular motor that utilizes the membrane potential of the cell (Kemp et al., 2004) to ratchet the T7 genome from …
The T7 ejection nanomachine components gp15-gp16 form a …
We show here that purified gp15 and gp16 can refold from a partially denatured state in vitro, and that gp15 interacts with gp16 to form a spiral ring structure. Purified gp15 binds to DNA, …
Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA …
2010年3月15日 · The latter adsorb and eject internal core proteins into the cell envelope but no phage DNA enters the cytoplasm. Extragenic suppressors of the defective gene 16 lie in gene …
Expression of Cpgp40/15 in Toxoplasma gondii: a Surrogate …
The glycoprotein products of the Cpgp40/15 gene, gp40 and gp15, are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for …
Cryo-EM structure of the periplasmic tunnel of T7 DNA ... - Cell Press
2021年8月5日 · Here, we reconstitute the phage T7 DNA-ejectosome components gp14, gp15, and gp16 and solve the periplasmic tunnel structure at 2.7 Å resolution. We find that gp14 …