2020年12月14日 · The authors propose the following model: GSC-produced Tkv is transported along the MT-nanotubes into the hub region at the interface between GSC and hub cells; Tkv binds hub cell-produced Dpp and initiates downstream signaling in GSCs; the Tkv/Dpp complex is subsequently translocated from GSC surface into hub cells and degraded in hub cells via ...

2015年7月7日 · MT-nanotubes are observed specifically within GSC populations, and require IFT (intraflagellar transport) proteins for their formation. The BMP receptor Tkv localizes to MT-nanotubes. Perturbation of MT-nanotubes compromises activation of Dpp signaling within GSCs, leading to GSC loss.

2018年9月11日 · Here, we demonstrate that Thickveins (Tkv), a type I bone morphogenetic protein (BMP) receptor, acts in the Drosophila developing ovarian soma through a Smad-independent pathway to shape the distribution of BMP signal within the niche, impacting germline stem cell (GSC) recruitment and maintenance.

2010年12月10日 · To understand the mechanism underlying the formation of a steep gradient of BMP response between GSCs and differentiated CBs, we used a transgene that expressed the constitutively active Dpp receptor, Tkv (ca) (Wieser et al., 1995), to explore the sensitivity of CBs to BMP signaling.

2019年10月17日 · Germaria with deficient Wg and Wnt6 in cap cells exhibited more GSC-like cells. They further provided evidence showing that these Wnts promote high expression level of Tkv in ECs where Tkv functions as a receptor sink to remove excess cap cell-produced Dpp, thus preventing passive diffusion of Dpp outside the niche.

In the Drosophila melanogaster female germline stem cell (GSC) niche, Decapentaplegic (Dpp), a fly transforming growth factor β molecule and well-established long-range morphogen, acts over one cell diameter to maintain the GSCs. Here, we show that Thickveins (Tkv; a type I receptor of Dpp) is highly expressed in stromal cells next to Dpp ...

2018年9月11日 · Here, we demonstrate that Thickveins (Tkv), a type I bone morphogenetic protein (BMP) receptor, acts in the Drosophila developing ovarian soma through a Smad-independent pathway to shape the distribution of BMP signal within the niche, impacting germline stem cell (GSC) recruitment and maintenance.

2019年1月1日 · Dpp/Gbb ligands in turn activate Thickveins (Tkv) and Saxophone (Sax) receptors in GSCs to specify GSC identity. Additionally, JAK-STAT signaling in the niche cells promote the production of Dpp to maintain GSCs (Decotto & Spradling, 2005; Lopez-Onieva, Fernandez-Minan, & Gonzalez-Reyes, 2008; Wang, Li, & Cai, 2008).

2004年5月1日 · GSCs are associated with terminal filament (TF) and cap cells (both somatic cells, dark blue). All GSCs and CBs contain spectrosomes (green dot) and express Dad - lacZ (blue dot). The CBs undergo four rounds of mitotic division with incomplete cytokinesis to produce 2, 4 and 8 germline cell cysts (CC) in Region 1 and 16 CC in Region 2A.

2018年2月27日 · These Wnts act redundantly to inhibit Dpp signaling outside of the GSC niche by upregulating the expression of Dpp receptor thickveins (tkv) in escort cells. Interestingly, Tkv expression in escort cells does not participate in signal transduction but instead acts as ‘receptor sink’ to prevent ectopic activation of Dpp signaling in the ...