gst-tag相对分子质量较大，约为26kd，插入在目的蛋白的c末端或n末端，大肠杆菌中常用在n端。gst(谷胱甘肽巯基转移酶) 蛋白本身是一个在解毒过程中起到重要作用的转移酶。一般选择gst标签的目的有两个，一是提高蛋白表达的可溶性，二是提高蛋白的表达量。

SDS-PAGE analysis of Human BMP-2 proteins, 2 μg/lane of Recombinant Human BMP-2 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 38 KD 实验操作视频 真核标签蛋白纯化操作视频

2024年6月28日 · GST pull-down： 一般用于体外实验，因为诱饵蛋白是需要加上GST标签的重组蛋白，所以是非生理状态下的验证，蛋白质的结构和形式可能与天然状态下存在一定差异， 一般用于体外验证两个已知蛋白的直接相互作用。

2024年7月24日 · 4）GST pull-down实验优缺点. GST pull-down优点： 1. 可验证蛋白质-蛋白质之间的直接互作反应，区别于Co-IP的复杂细胞背景，是体外验证蛋白质分子互作的经典方法； 2. GSH谷胱甘肽偶联球珠亲和力强，洗脱纯度高，能够分离出纯度更高的蛋白体系。 GST pull …

2018年5月4日 · GST-fused BGN constructs and its effect on BMP-2 function. (a) Schematic drawings of biglycan (BGN) core protein and deletion constructs including core protein without N and C termini; ∆NC, N terminal to leucine rich repeat domain (LRR)6; N-LRR6, LRR7 to C terminus; LRR7-C.Each shaded box represents LRR (L in the illustration) of BGN. (b) The presence of glutathione-S-transferase (GST ...

Our study characterizes the effects of GST-BGN on BMP signaling and functions by the C2C12 cell assay system and then tests the effect of GST-BGN on BMP-2-induced early osteogenesis in a rat mandible defect model. The hypothesis tested was that GST-BGN is a potent BMP-2 function promoter in vitro and in vivo, leading to predictable bone ...

2018年2月15日 · Recombinant Human BMP7 Protein, GST-tagged can be used for research. Creative BioMart to Present at BPS 2025 Annual Meeting | February 15-19, 2025 Learn ... Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. Source : ...

所使用的抗GST抗体可能能够与大肠杆菌某些蛋白结合，从而导致Western检测时出现杂带，选择经过大肠杆菌蛋白吸附过的抗GST抗体，就能很好地减少甚至消除这些杂带。 5. GST标签蛋白不能被完全酶切。 a. PreScission Protease，TEV Protease或Thrombin等酶的用量不正确。

BMP-7 Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 42.7 kDa and the accession number is P18075. 其他形式的 BMP-7 Protein, Human, Recombinant (GST):

Product information "BMP-2 Protein (Gst Tag) (recombinant human)" Sequence: Gln283-Arg396. Fusion tag: N-Gst Endotoxin: 1.0 EU per µg of the protein as determined by the LAL method Observed Molecular Mass: 42 kDa. Protein function: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins.