2015年7月3日 · Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4).

目的构建P33^ING1b核定位信号肽(NLS)与谷胱甘肽-S-转移酶(GST)重组的融合蛋白(GST-NLS)原核表达载体及建立GST-NLS纯化技术.方法先用RT-PCR从ING1基因中扩增编码NLS的cDNA片段,将其插入克隆质粒pbluescript-SK,再以克隆质粒NLS片段为模板,PCR扩增NLS cDNA片段,并加入限制性酶切位点 ...

名称：gst 描述：Glutathione S-transferase 应用注释：Facilitates purification by glutathione matrix and antibody binding of tagged protein; helps to stabilize tagged protein and protect it against intracellular protease cleavage.

1999年11月12日 · Protein shuttling between the nucleus and the cytoplasm is fundamentally controlled by both a nuclear localization sequence (NLS) 1 and a nuclear export sequence (NES). These topogenic sequences bind to specific receptors that allow selective passage through the nuclear pore complex (reviewed in Refs. 11, 12, 13).

1996年9月20日 · We show here that M9-mediated nuclear import occurs by a novel pathway that is independent of the well-characterized, importin-mediated classical NLS pathway. Additionally, we have identified a specific M9-interacting protein, termed transportin, which binds to wild-type M9 but not to transport-defective M9 mutants.

2008年9月22日 · To determine the patterns and amino acid residues responsible for nuclear import activity of an importin α-dependent classical NLS, we conducted a systematic amino acid replacement analysis for a bipartite NLS, which can yield a potentially stronger activity than the monopartite NLS (Hodel et al., 2001).

Purified GST-NLS-GFP was microinjected into the cytoplasm of Vero cells, and images were recorded at different time points post-injection using a charge-coupled device (CCD) camera. The nuclear...

2015年10月12日 · Structural and functional analyses identify key binding pockets on importin-α as potential targets for antiviral drug development. Unlike many other NLSs, NP ncNLS binds to the NLS-binding domain of importin-α weakly with micromolar affinity.

2018年3月28日 · 谷胱甘肽s-转移酶（gst） GST是最早被使用的表位标签之一; 它可以置于N或C端并可以蛋白质的可溶表达。 用谷胱甘肽结合树脂进行纯化。

2006年6月8日 · 摘要： 目的构建P33ING1b核定位信号肽(NLS)与谷胱甘肽-S-转移酶(GST)重组的融合蛋白(GST-NLS)原核表达载体及建立GST-NLS纯化技术.方法先用RT-PCR从ING1基因中扩增编码NLS的cDNA片段,将其插入克隆质粒pbluescript-SK,再以克隆质粒NLS片段为模板,PCR扩增NLS cDNA片段,并加入限制性酶 ...