The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique.

The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly... In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants.

GUS基因就是β-葡萄糖苷酸酶基因(β-glucuronidase)。 该基因产物为β-葡萄糖苷酸酶，属水解酶类，相当稳定而不易降解。 有简易的测定方法，且可使用市售的底物 (substrate) 在原位 (in situ) 显现出酶的活性，以肉眼即可检测其产物，非常方便。

The beta-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and computational analyses (including bioinformatics), its application in the study of plant gene expression is now an inte …

In molecular biology, β-glucuronidase is used as a reporter gene to monitor gene expression in mammalian and plant cells. Monitoring β-glucuronidase activity through the use of a GUS assay allows determination of the spatial and temporal expression of the gene in question.

GUS fusions are now widely used to study plant-pathogen and plant-symbiont interactions. They can be used both to study expression of particular genes and to mark and monitor populations of micro...

β-Glucuronidase (GUS) is a very versatile reporter of gene expression that is frequently used in plant molecular biology. The diverse applications of the GUS gene fusion systems (Gallagher, 1992) are based on the detection of the enzymatic activity of GUS in...

Factors influencing or mediating such regulation are often best studied using gene fusions. Gene fusions can be defined as DNA constructions (performed in vitro or in vivo) that result in the coding sequences from one gene (reporter) being transcribed and/or translated under the direction of the controlling sequences of another gene (controller ...

β-葡糖醛酸酶(gus)报告系统是现代分子生物学研究领域中被广泛使用的一种重要工具，在解析基因时空表达调控的研究中发挥着重要作用.本文概述了报告基因gus的生化特性及检测手段,从启动子元件鉴定、基因诱捕、无标记转基因技术等方面论述gus的应用现状和 ...

The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly referred to as the gus gene) has become one of the most widely used reporter genes in plant molecular biology.