姐妹染色单体交换（SCE）的检测原理及其分子机理 - 豆丁网
sce 具有 两 种特 殊 的 sce 增长 曲线 ：第一 种 曲线 ， SCE 频率达 一定 浓 度后 不 再 随处 理浓 度增 大 而 提 高 ；第 二 种 曲线 具 有一 个峰值 ，一定浓 度 时 SCE 频
姐妹染色单体互换试验 - 百度百科
姐妹染色单体互换试验（SCE）是检测化学物质所致的染色体同源位点上DNA复制产物互换频率的试验方法。 遗传学终点是原发性DNA损伤。 体外培养的 哺乳动物细胞 在有或无代谢活化的情况下与受试物接触，并在含有5-溴脱氧尿嘧啶核桃苷（BrdU）的培养液经历2个细胞周期，然后加入纺锤体抑制剂 (如秋水仙素)，收集细胞，制备染色体标本。 给啮齿类动物作受试物处理，再给以BrdU，处死前用纺锤体抑制剂，处死后，取骨髓细胞制备染色体标本。 标本经荧光 吉姆萨染 …
姐妹染色单体交换实验 - biomart.cn
2022年2月10日 · 姐妹染色单体交换（sister chromatid exchange，SCE）是指同一条染色体复制过程中形成的两条染色单体之间所发生的一类特殊的同源重组。 SCE 发生的机制尚不清楚，但其发生的频率可以反映细胞在 DNA 合成期的受损程度。
The sister chromatid exchange (SCE) assay - PubMed
A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination.
The Sister Chromatid Exchange (SCE) Assay - PubMed
A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination.
Telomere sister chromatid exchange and the process of aging
Telomeres are a hotspot for sister chromatid exchange (T-SCE). Any biological consequence of this form of instability remained obscure until quantitative modeling revealed a link between elevated T-SCE rates and accelerated cellular replicative ...
事前准备——姐妹染色单体交换（SCE）试验（C）（一）-新闻中 …
2015年11月3日 · sce是化学毒物引起的一种细胞遗传损伤。主要反映受试物导致染色体同源位点上dna复制产物互换频率的改变，sce频率可反映细胞在s期dna受损程度。
Sister chromatid exchange - Wikipedia
Sister chromatid exchange (SCE) is the exchange of genetic material between two identical sister chromatids. It was first discovered by using the Giemsa staining method on one chromatid belonging to the sister chromatid complex before anaphase in mitosis.
Sister Chromatid Exchange - an overview | ScienceDirect Topics
Sister chromatid exchange (SCE) is a natural phenomenon, which occurs frequently in multiplying somatic and germline cells, and has been observed in different cell lines, in mammalian and other animal and plant cells, both in vitro and in vivo. SCEs represent the reciprocal exchanges of DNA segments between sister chromatids of a chromosome at ...
姐妹染色体单体交换试验-新闻中心-标准物质网-河南普天同创计量 …
2017年3月17日 · 姐妹染色单体交换（sister chromatid exchange, SCE）是指在细胞周期的S期内的姐妹染色单体相同位点上的DNA片段发生互换，可能与DNA双链的断裂与复制有关，SCE的发生的频率可反映细胞在S期的受损程度。
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