When bound to Ca 2+, GCaMP fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. [3] It is used in biological research to measure intracellular Ca 2+ levels both in vitro and in vivo using virally transfected or …

GCaMP6f is a multistate uv fluorescent protein published in 2013, derived from Aequorea victoria.

2023年3月15日 · Using large-scale screening and structure-guided mutagenesis, fast and sensitive GCaMP sensors are developed and optimized with improved kinetics without compromising sensitivity or brightness.

2023年8月28日 · The extinction coefficient measures how effectively GCaMP absorbs light of a particular wavelength, which affects its ability to detect changes in the intracellular Ca2+ concentration. The extinction coefficient is measured in liters divided by mol per centimeter (L/mol–1 ∙ cm–1), or in molar units of extinction (M–1 ∙ cm–1).

For example, GCaMP excitation is blue (~470nm) and emission is green (~530nm). And this is where the second component is necessary. Dichroics and filters allow for the transmission of the correct excitation and emission wavelength to the imaging device.

GCaMP is a genetically encoded calcium indicator used in neuroscience research, consisting of a fusion protein of green fluorescent protein (GFP) and calmodulin. It increases fluorescence when bound to calcium ions, enabling live imaging of brain activity at a single-cell level.

2019年6月17日 · We present enhanced GCaMP sensors tailored to detect neuronal activity in specific applications. jGCaMP7 indicators include: jGCaMP7s (sensitive and slow), jGCaMP7f (fast kinetics), jGCaMP7b...

The pH titration fluorescence measurements are wavelength dependent. Using a more red shifted excitation wavelength for the pH-dependent fluorescence measurements (488 nm versus 470 nm, for example) limits the resolution of the multiple chromophore species that show up as pH increases, making it more difficult to determine individual pK a values.

By using one of the two beams as a reference and varying the wavelength of the second beam, we are able to determine precisely the wavelength dependence of ΔF/F in a live mouse brain. Considering the tissue absorption and GCaMP6s action cross section, we found that the optimal wavelength for 3PM of brain activity is around 1300 nm.

2023年2月22日 · We review the principles of development and deployment of genetically encoded calcium indicators (GECIs) for the detection of neural activity. Our focus is on the popular GCaMP family of green GECIs, culminating in the recent release of the jGCaMP8 sensors, with dramatically improved kinetics relative to previous generations.