
Ultrasensitive fluorescent proteins for imaging neuronal activity
2013年7月17日 · GCaMP6f is the fastest genetically-encoded calcium indicator for cytoplasmic free calcium in neurons, with sensitivity comparable to OGB1-AM (Fig. 1d–g).
GCaMP6f :: Fluorescent Protein Database
GCaMP6f is a multistate uv fluorescent protein published in 2013, derived from Aequorea victoria.
Fast and sensitive GCaMP calcium indicators for imaging neural
2023年3月15日 · In particular, the green fluorescent protein (GFP)-based GCaMP sensors 2, 3, 5, 6 have been iteratively engineered to enhance the signal-to-noise ratio (SNR) for detecting Ca …
什么是GCaMP6f?钙离子成像技术。 - CSDN博客
2022年6月22日 · GCaMP6f是一种基因编码的钙离子指示剂,常用于监测神经元活动。相较于GCaMP3,GCaMP6f的灵敏度提高了10倍,动力学快2倍,特别适用于检测快速钙信号变化 …
High-performance calcium sensors for imaging activity in neuronal ...
2019年6月17日 · Using structure-guided mutagenesis and neuron-based screening, we optimized the green fluorescent protein-based GECI GCaMP6 for different modes of in vivo imaging.
pGP-CMV-GCaMP6f - Addgene
Plasmid pGP-CMV-GCaMP6f from Dr. Douglas Kim's lab contains the insert GCaMP6f and is published in Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. This plasmid …
ER-GCaMP6f: An Endoplasmic Reticulum Targeted Genetic Probe …
ER-GCaMP6f identifies dynamics in calcium signaling of ER resident receptors that are missed by plasma membrane anchored indicators. We also generated an adeno associated virus (AAV5) …
CMV-Mito4x-GCaMP6f - Addgene
Plasmid CMV-Mito4x-GCaMP6f from Dr. Timothy Ryan's lab contains the insert 4 repeats of COX8 signal peptide plus GCaMP6f and is published in Neuron. 2019 Dec 10. pii: S0896 …
Imaging neuronal activity in the central and ... - PubMed Central …
Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of the Thy1.2 promoter. …
Structural basis of the ultrasensitive calcium indicator GCaMP6
GCaMP is one of the most widely used calcium indicators in neuronal imaging and calcium cell biology. The newly developed GCaMP6 shows superior brightness and ultrasensitivity to …
- 某些结果已被删除