030613 C57BL/6J-Tg (CAG-ARL13B/mCherry/GECO1.2)1Clph/J <em>Arl13b-mCherry-GECO1.2^tg</em> transgenic mice express a ratiometric calcium indicator in all ...

Optical imaging of calcium dynamics using genetically encoded calcium indicators (GECIs) is a powerful tool for systems neuroscience. The current state-of-the-art GECIs emit green light (green GECIs) and are derived from Green Fluorescent Protein (GFP).

2019年1月21日 · We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca 2+ imaging in combination with other optogenetic indicators and actuators. GECIs are often used together with optogenetic...

2022年3月1日 · We also describe a novel Calcium DoubleSpy mouse model that conditionally expresses both RCaMP in the cytoplasm and GEM-GECO1 in the mitochondrial matrix, allowing the study of mitochondrial Ca 2+ related physiology and pathogenesis simultaneously in two distinct intracellular compartments. 1.

2018年10月11日 · Here we present the generation and characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, driven by the Thy1 promoter. Four transgenic lines with different expression patterns showed sufficiently high expression for cellular in vivo imaging.

2018年10月11日 · Here we present the generation and characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, driven by the Thy1 promoter. Four transgenic lines with different expression patterns showed sufficiently high expression for cellular in vivo imaging.

2018年1月16日 · Genetically encoded calcium ion (Ca 2+) indicators (GECIs) are indispensable tools for measuring Ca 2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation.

2025年2月1日 · Here, we study the noninvasive multimodal fluorescence and optoacoustic imaging performance of state-of-the-art NIR calcium ion indicator NIR-GECO2G in the mouse brain. We observe robust in vivo signals with widefield fluorescence, and for …

2016年9月20日 · Genetically encoded Ca2+ indicators (GECIs) using fluorescent proteins are promising tools for organellar Ca2+ imaging, and much effort has been devoted to their development. In this review, we first discuss the key points of organellar Ca 2+ imaging and summarize the requirements for optimal organellar Ca 2+ indicators.

2014年11月15日 · We now report GCaMP-type low-affinity red fluorescent genetically encoded Ca2+ indicators for optical imaging (LAR-GECO), engineered to have Kd values of 24 μM (LAR-GECO1) and 12 μM (LAR-GECO1.2). We demonstrate that these indicators can be used to image mitochondrial and ER Ca2+ dynamics in several cell types.