2018年2月20日 · Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Using 15–20 µg protein per lane for mini- or midi-format gels is a good starting point for complex protein samples when staining with Bio-Safe™ Coomassie stain.

Figure 10 illustrates the visual information that can be obtained from an electrophoresis gel after it has run. The electric current uniformly moves all the DNA fragments through a gel in the same …

2015年8月26日 · GelJ is a user-friendly, platform-independent, open-source, and free tool that combines the simple design of free programs with instrumental features for the analysis of gel …

After fixing and staining the gel, typically with colloidal Coomassie, the gel lane is separated into equal gel slices and each slice is subjected to in-gel protease digestion followed by liquid …

DNA gel electrophoresis is a molecular biology technique for separating different sizes of DNA fragments. Applications of DNA gel electrophoresis include DNA fingerprinting (genetic …

If the commands Mark First Lane , Mark Next Lane and Plot Lanes are not shown in the Special menu then use Load Macros to open the file "Gel Plotting Macros" in the Macros folder. Use …

2012年4月1日 · In this paper, we proposed effective methods to track the center of and detect the lane as the first step to analyze PCR gel electrophoresis images. Previously proposed …

EasyLadder I is a ready-to-use DNA molecular weight marker, ideal for short runs (1cm to 3cm) on agarose gels. The ladder is pre-mixed with red loading buffer.

DNA migrates through the gel in a single, vertical lane. Three factors influence the speed of movement: (i) the voltage of the electrical field, (ii) the concentration of agarose, and (iii) most …