2020年11月18日 · We conducted detailed biophysical and crystallographic studies of the interaction between two G-rich DNA sequences, (GGGTT) 3 GGG (T1) and (TGGGT) 4 (T7), and a highly selective porphyrin ligand, NMM.

2020年11月18日 · Biological studies suggest that GQs may play roles in regulating gene expression, DNA replication, and DNA repair. Small molecule ligands were shown to alter GQ structure and stability and thereby serve as novel therapies, particularly against cancer.

2019年5月26日 · Biophysical and X-ray structural studies of the (GGGTT)3GGG G-quadruplex in complex with N-methyl mesoporphyrin IX. The G-quadruplex (GQ) is a well-studied non-canonical DNA structure formed by G-rich sequences found at telomeres and gene promoters.

2005年4月1日 · Mammalian telomeres end in single-stranded, G-rich 3′ overhangs resulting from both the “end-replication problem” (the inability of DNA polymerase to replicate the very end of the telomeres) and postreplication processing. Telomeric G-rich overhangs are precisely defined in ciliates; the length and the terminal nucleotides are fixed.

2024年3月15日 · Four-stranded G-quadruplexes (G4s) are DNA secondary structures that can form in the human genome. G4 structures have been detected in gene promoters and are associated with transcriptionally active chromatin and the recruitment of transcription factors and chromatin remodelers.

2021年2月4日 · We analysed the folding kinetics of human telomeric DNA (TA (GGGTTA) 3 GGGTT wtTel25) and TERRA RNA (UA (GGGUUA) 3 GGGUU TERRA25) G4s applying time-resolved NMR spectroscopy and introduced 19 F-modifications to modulate the structure and the folding kinetics of DNA G4.

2010年2月5日 · Human telomeric DNA and RNA G-rich sequences are capable of forming a four-stranded structure, known as the G-quadruplex. Such a structure might be important for telomere biology and a good target for drug design.

2013年10月10日 · We found that (GGGTT) 8 ssDNA was the most effective among the tested competitors, which include (GTTGT) 8, (GTTTT) 8, and (GTGTGT) 7 ssDNA (Fig. 2, A and B, and Table 1). Furthermore, we detected the complex formation between ORC and (GGGTT) 8 or (GGGAA) 8 ssDNA ( lanes 2 , 3 , 17 , and 18 , Fig. 2 C ).

2020年11月18日 · In this work, we investigate the interaction of a G-rich sequence, 5’-GGGTTGGGTTGGGTTGGG-3’ (T1), with a water-soluble porphyrin, N-methyl mesoporphyrin IX (NMM) via biophysical and X-ray crystallographic studies.

2021年7月2日 · In the present study, we demonstrate that longer GGGCTA arrays fold into G4 and into hairpins. We also demonstrate that replication protein A (RPA) efficiently binds to GGGCTA repeats structured into G4 but poorly binds to …