2003年4月10日 · Here we describe the cloning and sequencing of the full-length genome of GHPV, which revealed an organisation typical of polyomaviruses. Alignments of the amino acid sequences deduced from the major ORFs showed homologies to large T, small t, VP1, VP2, and VP3 of other polyomaviruses.

2023年1月26日 · Goose haemorrhagic polyomavirus (GHPV) is the viral agent of hemorrhagic nephritis and enteritis of geese (HNEG), a lethal disease of goose. The study describes the results of a molecular analysis Polish isolates of GHPV from geese and free-living birds based on complete VP1 gene and VP2 gene sequences.

2020年11月20日 · One (H31), two (H4–H5) and four (H27–H30) haplotypes were identified in Germany, China and France, respectively (Figure 2 (B)). Many divergent haplotypes were identified in Hungary (H6–H26). The haplotype network suggested that Taiwanese GHPV was closely related to German and Hungarian GHPV (Figure 2 (B)).

Routine culturing of goose haemorrhagic polyomavirus (GHPV) is cumbersome, and limited data are available about its replication and gene expression profile. In this study, goose embryo fibroblast cells were infected with GHPV for temporal measurement of the viral genome copy number and mRNA levels with quantitative PCR.

GHPV HUN-01, isolated from the liver of infected Landes geese, shared a close evolutionary relationship with the Toulouse Goose 2000 and 14234 strain, isolated from geese in France and Hungary. The isolation of GHPV from the livers of dead embryos also demonstrates that the virus can be transmitted vertically.

In this study, goose embryo fibroblast cells were infected with GHPV for temporal measurement of the viral genome copy number and mRNA levels with quantitative PCR.

2014年2月11日 · Here we describe the genome sequence and structure of GHPV of a goose from a Hungarian goose flock showing characteristics of the haemorrhagic nephritis and enteritis. The GHPV genome investigated in this study was 5252 bp long and was very similar (99% nucleotide identity) to sequences deposited in the GenBank.

2 Summary Goose hemorrhagic polyomavirus (GHPV, or Anser anser polyomavirus 1) is a small dsDNA virus that may cause fatal disease in young geese. In this study, GHPV positivity was examined in goose and duck samples collected in Hungary between 2005 and 2019. In this

Due to low doses of infection, an efficient and sensitive virus detection method is necessary to detect low amounts of goose hemorrhagic polyomavirus (GHPV). In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses.

2020年10月6日 · Overall, the findings demonstrate that GAstV infection causes renal damage by inducing renal cell apoptosis, fibrosis, and excessive inflammatory response, which subsequently leads to hyperuricemia and lethal visceral gout formation.