Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence or a gene on a chromosome within a person’s genome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.

基因组 原位杂交(Genome in situ hybridization，GISH)技术是20世纪80年代末发展起来的一种原位杂交技术。 它主要是利用物种之间 DNA同源性 的差异，用另一物种的 基因组DNA 以适当的浓度作封阻，在靶染色体上进行 原位杂交 。

Fluorescent in situ hybridization (FISH) is a powerful cytogenetic technique for identifying chromosomes and mapping specific genes and DNA sequences on individual chromosomes. Genomic in situ hybridization (GISH) and multicolor FISH (mc-FISH) represent two special types of FISH techniques.

2022年3月24日 · 荧光原位杂交（Fluorescence in situ hybridization FISH）是一门新兴的分子细胞遗传学技术，是 20 世纪 80 年代末期在原有的放射性原位杂交技术的基础上发展起来的一种非放射性原位杂交技术。

FISH： 采用了已知序列的、特异性的单链核酸作为 探针，标记了生物素或荧光素，在一定的温度和离子浓度下通过 碱基互补配对 法则，使得DNA-DNA原位杂交，采用荧光法显示，最终将DNA在细胞爬片、组织切片 (石蜡切片or冰冻切片)的原始位置上标记出来的一种技术。 上一代的FISH还是采用的同位素进行标记，现在基本上都是荧光标记了。 在此，要感谢J.G.Baunlan这位大神了。 有了FISH，我们可以不再仅仅依赖分子生物学的方法来检测DNA的表达了。 FISH技术最大的优 …

2015年6月30日 · In situ hybridization techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), can identify chromosome morphologies and sequences, amount and distribution of various types of chromatin in chromosomes, and genome organization during the metaphase stage of meiosis.

GISH technique follows the same protocol as the fluorescent in situ hybridization (FISH) technique. However, genomic and blocking DNA utilization in GISH differentiate it from FISH analysis. In plant species, genome organization and homology study are carried out by the use of genomic in situ hybridization (GISH).

2019年3月2日 · FISH and GISH are powerful cytogenetic techniques that allow the detection and localization of specific DNA sequences on chromosomes. FISH uses fluorescent probes to visualize DNA locations, while GISH uses total genomic DNA as probes.

Fluorescence in situ hybridization (FISH) is the most convincing technique for locating the specific DNA sequences, diagnosis of genetic diseases, gene mapping, and identification of novel oncogenes or genetic aberrations contributing to various ...

赛默飞的原位杂交 (ish) 是一种强大的技术，用于定位固定组织和细胞中的特定核酸靶标，提供与基因表达和遗传位点相关的时间和空间信息。我们的ish技术包括荧光 (fish) 和显色 (cish) 检测，适用于不同的应用需求。了解更多信息，探索我们的产品和服务。