In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly (vinyl alcohol) (PVA-COOH) to...

2015年5月6日 · We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer...

2008年5月15日 · The UV–Visible studies show that GOX crystals can be used for nonlinear applications. The dielectric measurement indicates that the GOX crystals have domains of varying sizes and varying relaxation time.

2016年8月15日 · We demonstrated that GOx were covalently attached to MWCNTs, without physical adsorption, by optimizing the EDC/NHS-mediated immobilization and washing step. The covalently bound GOx amounted to 3.3 mg-GOx/mg-MWCNTs, which is the highest achieved value reported so far.

2013年3月15日 · The π–π stacking interaction operating between the MWCNT and graphene oxide (GO) has been revealed by UV–Vis absorption spectroscopy. GOx was well immobilized onto the ERGO–MWCNT hybrid film, as a result direct electrochemistry of …

Glucose typically achieves high conversion rates with the aid of glucose oxidase (GOx) enzymes or by fermentation. GOx is subjected to degradation, possesses poor recyclability and operates...

Ultraviolet-visible (UV-Vis), fluorescence, and circular dichroism (CD) spectroscopies were applied to evaluate changes arising from the presence of nanomaterials both in the overall structure of GOx, as well as in the FAD moiety of the enzyme, which is responsible for its oxidation activity.

In this study, we reported the preparation of a conducting polymeric/inorganic nanohybrid consisting of multiwalled carbon nanotubes (MWCNT), N-doped graphene (NGr), and poly...

2024年10月16日 · 荧光素标记葡萄糖氧化酶 (GOx-FITC) 是一种结合了葡萄糖氧化酶（GOx）的催化活性与荧光素异硫氰酸酯（FITC）的荧光特性的新型标记酶。 通过共价键合的方式，将FITC与GOx的氨基残基进行反应。 FITC中的异硫氰酸酯基团能够与酶分子中的自由氨基（主要是赖氨酸残基）形成稳定的共价键。 通常在碱性条件下进行反应（pH 8.0-9.0），以保证氨基基团的活性。 使用透析或凝胶过滤的方法去除未反应的FITC分子，以得到高纯度的GOx-FITC复合物。 使用 …

2017年11月22日 · Luminol, horseradish peroxidase (HRP), and glucose oxidase (GOx) ternary functionalized graphene oxide (HRP/GOx-luminol-GO) with excellent chemiluminescence (CL) activity and specific enzymatic property was prepared via a …