Visualizing bacterial DNA replication and repair with molecular ...
Importantly, single-molecule microscopy can be easily applied to bacteria in vivo [39-41], making it now possible to visualize DNA replication and repair in real time in living bacterial cells. In this review, we provide an overview of some recent studies on bacterial DNA replication and repair using single-molecule microscopy.
A bacterial expression cloning screen reveals single-stranded DNA ...
2024年11月11日 · Sequencing the populations of surviving bacteria revealed enrichment of mitochondrial single-stranded DNA-binding proteins (mtSSBs) from both tardigrade species. Expression of mtSSBs in bacteria improved desiccation survival as strongly as the best tardigrade protectants known to date.
Proksee: in-depth characterization and visualization of bacterial ...
2023年5月4日 · Proksee (https://proksee.ca) provides users with a powerful, easy-to-use, and feature-rich system for assembling, annotating, analysing, and visualizing bacterial genomes. Proksee accepts Illumina sequence reads as compressed FASTQ files or pre-assembled contigs in raw, FASTA, or GenBank format.
MicrobiologyOpen - Wiley Online Library
By extracting DNA from sound and decayed wood samples at various stages of natural decomposition in both conifer (gymnosperm) and angiosperm wood substrates, we found that DNA quality significantly impacted the ability to amplify and sequence the samples.
Article Multiscale Structuring of the E. coli ... - ScienceDirect
2018年2月8日 · Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts: the replication terminus (ter) region and the rest of the chromosome.
Targets for repair: detecting and quantifying DNA damage with ...
Developments in these fluorescence-based strategies are reviewed here. Graphical Abstract. DNA adducts are induced endogenously via the production of reactive metabolic byproducts and during cellular propagation, and exogenously through environmental exposures to DNA damaging chemicals and radiation.
We describe a method for simultaneously depleting DNA from intact human cells and extracellular DNA (human and bacterial) in sputum, using selective lysis of eukaryotic cells and endonuclease digestion.
Combination of direct boiling and glass beads increases the purity …
2023年7月18日 · Abstract Extraction of DNA is a key step in molecular biology experiments and important for counting tiny microbial individuals. Direct boiling and mechanical cell lysis like glass beads are two in...
Determination of bacterial DNA based on catalytic oxidation of …
2018年8月11日 · Graphical abstract Schematic presentation of a colorimetric assay for bacterial DNA. It is based on the catalytic activity of a G-quadruplex DNAzyme that is formed by an asymmetric PCR involving triplex DNA formation and gold nanoparticle (AuNPs) aggregation.
Optimized bacterial DNA isolation method for microbiome …
Here, we present a DNA isolation protocol for tissue, optimized on biopsies from resected human colons (~2–5 mm in size), which includes reduction of human DNA without distortion of relative bacterial abundance at the phylum level.