2018年10月8日 · These channelrhodopsins, named GtACR1 and GtACR2, have near-perfect anion selectivity and produce large photocurrents in mammalian cells, owing to a higher single-channel conductance than that of...

2021年4月6日 · 比如阴离子通道模式的channelrhodopsins(GtACR1, Guillardia theta anion channel rhodopsin 1)。最敏感的波长也大概是470nm左右，会选择性地让各种阴离子通过，比如Cl-，从而超极化膜电位抑制到神经元。

尽管不是很常用，光激发的阴离子通道GtACR1和GtACR2在光敏感性上比氯泵更有优势，0.1 mW以下的激光能量也能引起较大的电流。 这使得GtACRs和SwiChR++ （一种仅由短脉冲光照就可引发长时间的电流响应的双稳定阴离子通道） 成为避免长时程光抑制对脑组织加热的有 ...

2017年10月23日 · Here, we explore the utility of the recently discovered anion channelrhodopsins GtACR1 and GtACR2 for application in the visual system of Drosophila. We first characterized their properties using...

2017年1月23日 · Here we show that both GtACR1 and GtACR2 potently, rapidly and reversibly inhibit a range of behaviors in Drosophila during controlled exposure to light. We expressed genes encoding the GtACRs in...

2023年2月8日 · Guillardia theta anion channelrhodopsin 1 (GtACR1) is a widely used inhibitor of optogenetics with unique conductance mechanisms and photochemistry. However, the molecular mechanism of light-gated anion conduction is poorly understood without a crystal structure for the intermediate state.

2021年8月29日 · These shortcomings may be mitigated by using new optogenetic proteins like Guillardia theta Anion Channelrhodopsin (GtACR1), which produces a repolarizing outward current upon illumination. Accordingly, we designed a study to assess the feasibility of GtACR1-based optogenetic arrhythmia termination in human hearts.

2024年4月16日 · Anion channelrhodopsin Gt ACR1 is a powerful optogenetic tool to inhibit nerve activity. Its kinetic mechanism was interpreted in terms of the bacteriorhodopsin photocycle, and the L intermediate was assigned to the open channel state.

The simulation results showed that GtACR1-based optically paced CM displayed optogenetic voltage forcing and CaTnC kinetics weakening, and light intensity inhibited myocardial electromechanical activity more than light duration. Our findings suggest that GtACR1 plays an important role of optogenetic modulation on CM electromechanical properties.

Anion channelrhodopsin GtACR1 is a powerful optogenetic tool to inhibit nerve activity. Its kinetic mechanism was interpreted in terms of the bacteriorhodopsin photocycle, and the L intermediate was assigned to the open channel state.