2024年9月19日 · Here, we use a lipid mixing assay based on Förster resonance energy transfer (FRET), and single-vesicle characterization approaches to identify that sub-micron-sized vesicles are induced to fuse by...

GUVs are more useful as artificial cell models for eukaryotic cells due to similarity in size. Additionally, their size enables observation of membrane domain structure using light microscopy. Here, we will focus on the electroformation method in the context of GUV formation.

2023年8月1日 · Giant unilamellar vesicles (GUVs) are cell-membrane mimetic tools. GUV preparation techniques and formation mechanisms are discussed that determine vesicle properties. Immobilization strategies of GUVs are summarized. Key membrane-biophysics and bottom-up biology applications of GUVs are highlighted.

2015年12月1日 · Giant unilamellar vesicles (GUVs) are simple model membrane systems of cell-size, which are instrumental to study the function of more complex biological membranes involving heterogeneities in lipid composition, shape, mechanical properties, and chemical properties.

2024年8月16日 · Here, we demonstrate three-dimensional (3D) reconfigurable DNA origami pincers (DOPs) that multitask on giant unilamellar vesicles (GUVs) for dynamic GUV remodeling, capturing, compartmentalization, and subsequent detachment of lipid fragments from GUVs, as well as transport of molecules across the membrane by inducing transient pores . In the ...

For encapsulating water-soluble materials and/or water-dispersible particles or functionalizing membrane proteins and/or other synthesized amphiphiles, giant unilamellar vesicles (GUVs) have been applied in various fields, such as supramolecular chemistry, soft matter physics, life sciences, and bioengineering.

Here, the gradual tension-mediated growth of cell-sized (≈10 µm) giant unilamellar vesicles (GUVs) is demonstrated, to which nanometer-sized (≈30 nm) small unilamellar vesicles (SUVs) are provided, that act as a lipid source.

2013年4月15日 · GUVs with FhuA, a bacterial porin, were used to follow the DNA injection by T5 phage upon binding to its transmembrane receptor. GUVs incorporating BmrC/BmrD, a bacterial heterodimeric ATP-binding cassette efflux transporter, were used to demonstrate the protein-dependent translocation of drugs and their interactions with encapsulated DNA.

脂质体-纳米粒是一种新型的纳米药物载体，是由 脂质体 包裹纳米粒自组装形成的核-壳结构， 巨型单层囊泡是山单层 磷脂双分子层 形成的壳状结构的囊泡，它处于水环境中，尺寸一般较大，百径约为 5-2004m，因此可以用 光学显微镜 直接观察其形貌。 巨型囊泡的尺寸与真实细胞较接近，且保留了细胞的很多物理化学性质，而且它的形状和成分可以根据实际情况进行调节和控制，因此它是一种很理想的生物膜的模型。 巨型单层囊泡已经很地用于研究生物膜的相分离、内乔 …

高分辨透射电子显微术 (high resolution transmission electron microscopy，简称HRTEM或HREM)是 相位衬度 (高分辨电子显微像的衬度是由合成的投射波和衍射波之间的相位差形成的，称为相位衬度 (phase contrast)。 ) 显微术，它能得到大多数晶体材料的原子排列像。 高分辨率透射电子显微术始于20世纪50年代，1956年 J.W.Menter 用分辨率为8 Å透射电子显微镜直接观察到酞菁铜间距为12Å的平行条纹，开启了之后的高分辨电子显微术的大门。 到20世纪70年代 …