2013年7月2日 · We first demonstrate the efficiency of GUV formation on a PVA gel and study the growth process by inspecting the distribution of lipids before and after growth. Next, we compare growing efficiencies for different ionic and nonionic amphiphiles and show that the method allows us to encapsulate hydrophilic substances in the vesicles.

2023年8月1日 · Giant Unilamellar Vesicles (GUV) are excellent membrane models and scaffolds to mimic cells and their functions. Their diverse and exciting applications largely depend on GUV properties imparted by their preparation techniques. This review outlines the advances made in the preparation techniques and discusses the formation mechanisms.

2017年11月20日 · Giant Unilamellar Vesicles (GUV) are the most commonly used biomimetic membrane model. Their production in physiological conditions through the well-known electroformation process is complex and other gel-assisted hydration procedures have recently emerged to circumvent some limitations of the process.

2018年8月7日 · Giant unilamellar vesicles (GUVs) are increasingly used as a versatile research tool to investigate membrane structure, morphology and phase state. In these...

Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.

2020年3月16日 · In the method described here, we generate large numbers of free-floating GUVs through the rehydration of lipid films deposited on soft polyacrylamide (PAA) gels. We show that this technique...

巨型单层囊泡（giant unilamellar vesicles，GUVs），指的是和细胞差不多大小的脂质泡，其外表的脂膜可以用来模拟细胞膜，也可以称之为仿生囊泡，能用于人造细胞方面的研究，那是个“高级”研究，不过本文要说的是更常见的药物筛选领域。 （用微流控技术生成细胞大小囊泡示意图） 传统方法做的囊泡，往其脂膜添加一些生物分子（如Min蛋白）后，会导致囊泡破裂。 但在2016年，科学家基于微流控技术已将其很好地攻克，使用的方法叫OAL法（辛醇辅助脂质体组装方 …

该研究是在由磷脂（POPC 和 DPPC）或基于聚（二甲基硅氧烷）和聚（环氧乙烷）的两亲共聚物制成的 GUV 上进行的。 与经典电铸工艺相比，通过 PVA 凝胶辅助水化方法形成的 GUV 观察到拉伸模量、横向扩散系数和膜堆积的显着偏差。 我们能够证明这种偏差是由于膜和悬浮介质中存在 PVA 链。 在全球范围内，我们的研究结果表明，必须特别注意凝胶辅助水合过程，因为获得的囊泡呈现出改变的机械行为。 摘要 巨型单层囊泡（GUV）是最常用的仿生膜模型。 它们通过众 …

2024年12月10日 · In the process, we also developed a treatment for recycling agarose and polyvinyl alcohol hydrogels for GUV production, and successfully encapsulated porcine liver esterase (PLE-GUVs). PLE-GUVs offer a novel method of GUV labelling and tracing, which emulates the calcein-AM staining behaviour of cells.

2019年1月21日 · 近日， 哈尔滨工业大学韩晓军 教授（点击查看介绍）团队 结合微接触剥离技术和电形成方法制备了巨型磷脂囊泡（guv）阵列，并在阵列中载入羧基荧光素（cf）、dna和hrp等生物分子，构建单室人造细胞阵列，来研究物质的跨膜运输和细胞新陈代谢功能 。