In vitro analysis using purified components directly confirmed that GcvB binds to rbn mRNA in the presence of Hfq and that RNase E cleaves rbn mRNA. These data identify a novel mechanism for regulation of RNase BN and add to the growing list of diverse processes that …

2009年1月1日 · In Escherichia coli, the gcvB gene encodes a nontranslated RNA (referred to as GcvB) that regulates OppA and DppA, two periplasmic binding proteins for the oligopeptide and dipeptide transport systems.

2021年11月5日 · Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in …

We investigated small RNA (sRNA) clients of Hfq, an RNA chaperone that promotes mutagenic break repair (MBR), and found that GcvB promotes MBR by allowing a robust σ S response, achieved via opposing the membrane stress (σ E) response.

2022年8月20日 · GcvB is one of the most conserved sRNAs that is regarded as the master regulator of amino acid uptake and metabolism in a wide range of Gram-negative bacteria. However, little information about GcvB-mediated regulation in V. alginolyticus is available.

2009年1月1日 · In Escherichia coli, the gcvB gene encodes a small non-translated RNA that regulates several genes involved in transport of amino acids and peptides (including sstT, oppA and dppA).

2009年1月1日 · Hfq, an RNA chaperone protein, binds many small RNAs and is required for the small RNAs to regulate expression of their respective target genes. We showed that repression by GcvB of phoA translational fusions is dependent upon Hfq.

2008年4月1日 · The gcvB gene encodes two small, nontranslated RNAs that regulate OppA and DppA, periplasmic binding proteins for the oligopeptide and dipeptide transport systems. Analysis of the gcvB sequence identified a region of complementarity near the ribosome-binding sites of dppA and oppA mRNAs.

GcvB is one of the most highly conserved Hfq-associated small RNAs in Gram-negative bacteria and is known to repress several ABC transporters for amino acids.

2015年1月28日 · We show that mRNA decay from the gltIJKL locus encoding an amino acid ABC transporter generates a stable fragment (SroC) that base‐pairs with GcvB. This interaction triggers the degradation of GcvB by RN ase E, alleviating the GcvB‐mediated mRNA repression of other amino acid‐related transport and metabolic genes.