2025年1月15日 · Knocking out the enzyme gamma glutamyl carboxylase (GGCX) reduces virus replication in vitro and in vivo by inhibiting the carboxylation modification of viral haemagglutinin (HA) and the adhesion...

2024年10月31日 · Intercellular cross-talk analysis highlighted enhanced interactions among bystander immune cells during H1N1 infection, with neutrophils in humans and macrophages in mice showing the most remarkable increases in interaction intensity.

2021年9月14日 · In this study, we show that glycolysis is upregulated in H1N1-infected human lung epithelial (A549) cells and mouse lung tissues and that inhibiting this metabolic pathway results in reduced H1N1 replication. Furthermore, the effect of glycolytic inhibitors or enhancer on H1N1 infection is independent of interferon signaling.

Inhibition of the Shh pathway restores the levels of Snail and intercellular junction proteins in H1N1-infected cells. Our study suggests that Gli1 activation plays an important role in disrupting the intercellular junctions and in promoting the pathogenesis of H1N1 virus infections.

2024年2月1日 · HIF-1α is one of the major host molecules that facilitates H1N1 replication and promotes cytokine storm. Mortality was decreased in HIF-1α knockdown mice infected with H1N1 compared to wild-type H1N1-infected mice. This study explores the mechanism of HIF-1α, which regulates glucose metabolism reprogramming in alveolar epithelial cells.

Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic H1N1 (A/Texas/05/2009), and highly pathogenic avian influenza H5N1 (A/Thailand/KAN-1/2004) viruses on mouse myeloid den...

The kinetics of CXCL10 gene expression in the 2009 A (H1N1) virus-infected cells was, however, slower than that induced by seasonal H1N1 or H3N2 viruses. This also led to lower secretion of CXCL10 protein from pandemic H1N1 virus-infected DCs. It is unclear whether the delayed production of NK and Th1 cell-recruiting CXCL10 in DCs has an effect ...

3 天之前 · We studied the breadth of binding antibody responses to related pandemic-lineage H1N1 influenza viruses at 8 weeks after immunization. We obtained comparable HA-specific binding antibody titers to H1N1 A/Michigan/45/2015, A/Wisconsin/588/2019, A/Sydney/5/2021, and A/Victoria/4897/2022 (Figure 5K) with all 3 vaccine platforms.

2025年2月21日 · All antibody products were acquired from Biolegend unless otherwise specified. For antigen-specific B cell staining, cells were initially labeled for 30 minutes on ice with (2μg/ml) biotinylated A/California/07/2009 (H1N1) hemagglutinin (HA) protein (Immune Technology), before washing three times with Staining Buffer to remove unbound HA.

2019年3月14日 · In this study, we identified a fully human H1N1 neutralizing antibody (32D6) via an Epstein-Barr virus-immortalized B cell-based technology. 32D6 specifically neutralizes the clinically...