To study such specificities, we developed a diverse set of HA-domain proteins based on an H1N1 pdm2009-like influenza virus strain, including monomeric head and trimeric stem domain, as well as the full HA-trimer. These proteins were used to study the B cell and antibody responses in six healthy human donors.

2018年7月11日 · Here we analyze both evolutionary rates and positive selection of the head and stalk domains of HAs from pre-pandemic seasonal H1N1 (sH1N1), pandemic H1N1 (pH1N1), H3N2 and the two...

There are 18 known HA subtypes (H1–H18) and 11 known NA subtypes (N1–N11). HA subtypes are further divided into two groups. Group 1 HA includes H1, H2, H5, H6, H8, H9, H11, H12, H13, H16, H17, and H18, whereas group 2 HA includes H3, H4, H7, H10, H14, and H15. Both influenza A and B viruses infect humans and can cause severe illness or death.

2019年4月3日 · Here, we quantified H1N1 HA head- and stalk-specific Abs in 179 adults hospitalized during the 2015–2016 influenza virus season. We found that HA head Abs, as measured by hemagglutinin inhibition (HAI) assays, were associated with protection against naturally acquired H1N1 infection.

2021年7月2日 · The head-directed mAb, targeting an epitope distinct from known head-specific mAbs, showed relatively broad H1N1 neutralization and the stem-directed mAb was able to broadly neutralize diverse H1N1 viruses.

2010年7月12日 · The main findings in the current study are: (a) bacterially expressed H1N1 HA1 (1–330) and HA (1–480) can be purified as properly folded proteins as determined by CD spectroscopy, SPR analyses with H1N1 immune sera, and adsorption of neutralizing activity from post-infection and post vaccination sera; (b) the HA1 (1–330) contained >50% ...

Here, we reveal first exposure to the 2009 pandemic H1N1 influenza virus recalls memory B cells that are specific to the conserved receptor-binding site (RBS) or lateral patch epitopes of the hemagglutinin (HA) head domain.

2016年4月18日 · We performed an epitope mapping experiment with human immune sera from a clinical trial with a monovalent live-attenuated influenza virus vaccine (NC99, H1N1) and synthetic NC99 HA peptides and could show that immunisation with a LAIV can induce antibodies that bind to synthetic peptide targets located both in the HA head and stalk domain (Fig ...

2021年6月2日 · Here, we reveal that first exposure to the 2009 pandemic H1N1 influenza virus recalls memory B cells that are specific to the conserved receptor-binding site (RBS) or lateral patch epitopes of the hemagglutinin (HA) head domain.

Influenza A virus subtype H1N1 (A/H1N1) is a subtype of influenza A virus (IAV). Some human-adapted strains of H1N1 are endemic in humans and are one cause of seasonal influenza (flu). [1] Other strains of H1N1 are endemic in pigs (swine influenza) and in birds (avian influenza). [2]