2018年12月18日 · We tracked IFN-γ and IL-12p40 in vivo during rejection of aPD-1 treatment-sensitive MC38 tumor cells, which were labeled with H2B-mApple. We also tracked macrophages, which were tagged with Pacific-blue-dextran nanoparticles (Weissleder et al., 2014), as these cells are often abundant in tumors (Engblom et al., 2016).

Stable cancer cell lines, MC38 (H2B-RFP), MC38 (H2B-RFP/Cre, B16F10 (H2B-RFP), and B16F10 (H2B-RFP/Cre), were generated by retroviral transduction using pBABE-based retroviruses, and polyclonal populations were selected by antibiotic resistance and flow-sorted for bright fluorescence as appropriate.

MC38 cancer cells expressing H2B-mApple were implanted into the reporter mice, and tumors and other organs were harvested after 7 days. Animal tissues were subsequently analyzed with a panel of immune cell markers for Arg1 expression by flow cytometry (Figure 1 B).

2018年5月21日 · Here, we show that R848, an agonist of the toll-like receptors TLR7 and TLR8 identified in a morphometric-based screen, is a potent driver of the M1 phenotype in vitro and that R848-loaded β...

2024年3月12日 · We implanted MC38-H2B-mApple tumor cells in IL12-eYFP reporter mice, which were treated with Pacific Blue-dextran as a macrophage-imaging agent to allow for the simultaneous visualization of tumor cells (mApple), TAMs (Pacific Blue) and TAM phenotype (co-expression of IL-12 and eYFP), respectively.

2021年6月21日 · We transferred NFAT-GFP-expressing Treg cells into BMCs 3 weeks after bone marrow transfer, when the T cell compartment was not yet reconstituted, in order to enhance their engraftment. Another 3 weeks later, we implanted MC38 H2B-Cer tumors, installed DSFC, and treated animals either with DT or vehicle (Figure 2 A).

2021年11月7日 · 方法 将小鼠结肠癌MC38细胞移植到24只C57BL/6J小鼠皮下，荷瘤后每周一次记录小鼠体质量及肿瘤大小的变化，每周一次采用流式细胞术检测肿瘤浸润免疫细胞水平，包括 CD45 + 细胞、CD3 + T 细胞、CD8 + T 细胞、CD4 + T细胞、自然杀伤（natural killer，NK）细胞 …

2024年12月2日 · 该细胞为已经稳定转染GFP的细胞，随细胞传代次数的增加，其GFP荧光强度会逐渐减弱。 若实验要求需要维持荧光强度，可以加入嘌呤霉素进行再次筛选。 1)收到细胞后，75%酒精消毒瓶壁将T25瓶置于室温放置约1h，若发现培养瓶破损、有液溢出及细胞有污染，请拍照后及时联系我们。 2)请在4或5X显微镜下确认细胞状态，同时给刚收到的细胞拍照（10×，20×）各2-3张以及培养瓶外观照片一张留存，作为售后时收到时细胞状态的依据。 3) …

2022年7月14日 · 磁共振成像（magnetic resonance imaging, MRI）和超声等临床成像技术能够对癌症的位置、解剖结构及其随时间的变化情况进行宏观测量，但无法测量病变的细胞和分子尺度细节。 活体显微（intravital microscopy, IVM）和光学相干断层扫描（optical coherency tomography, OCT）采用不同的成像机制，能达到亚细胞尺度的分辨率，促进临床和临床前的广泛研究（图1 ）。 图1. IVM、OCT和标准临床成像模式间，空间分辨率与成像深度的对比示意图。

We implanted 1 million MC38-H2B-mCherry colon adenocarcinoma cells into the tissue underlying the window chamber to facilitate host immune cell recruitment. Within 24–72 h of MC38-H2B-mCherry cell implantation, we discovered host Csf1r Cre GCaMP5 fl reporter cells with highly dynamic calcium elevations.