HCV genome Nonstructural protein 3 ( NS3 ), also known as p-70 , is a viral nonstructural protein that is 70 kDa cleavage product of the hepatitis C virus polyprotein. It acts as a serine protease .

Study of NS3 protease activity has been very practical, focusing on the pursuit of inhibitors as specifically targeted antiviral therapy for hepatitis C (STAT-C). Study of NS3 helicase activity has been much more fundamental, focusing on the use of this helicase as a model for the structure and mechanism of DE x H proteins in general.

2022年8月4日 · NS3蛋白酶抑制剂波普瑞韦（boceprevir）和特拉匹韦（telaprevir）是2011 年5月批准用于与聚乙二醇干扰素和利巴韦林联合治疗HCV患者的首个HCV直接作用抗病毒药物。

The serine-type protease region of NS3 is responsible for most of the cleavages of the viral polyprotein that free nonstructural proteins. In addition, NS3 plays a role in subverting the innate host immune response by proteolytically inactivating various …

Hepatitis C virus (HCV) is a major cause of liver-related diseases and hepatocellular carcinoma. The helicase domain of one of the nonstructural proteins of HCV, NS3 (nonstructural protein 3), is essential for viral replication; however, its specific biological role is still under investigation.

2009年6月23日 · In order to study the humoral immune responses against different HCV proteins in patients suffering from chronic HCV infection, we produced three structural (core, E1 and E2) and six nonstructural proteins (NS2, NS3, NS4A, NS4B, NS5A and NS5B) in Sf9 insect cells by using the baculovirus expression system.

The C-terminal portion of hepatitis C virus (HCV) nonstructural protein 3 (NS3) forms a three domain polypeptide that possesses the ability to travel along RNA or single-stranded DNA (ssDNA) in a 3′ to 5′ direction.

NS3 丝氨酸PR 是HCV非结构蛋白加工成熟过程的关键酶，与三磷酸核苷酸酶、RNA 解螺旋酶 结合，构成HCV 复制所必需的非结构蛋白NS3。 NS3 属于胰PR 超家族，拥有PR 和RNA 解螺旋酶双重活性，位于由HCV RNA 编码的前体多聚蛋白的 开放阅读框 (Open reading frame，ORF) 中的非结构蛋白区中。 另外，NS3/4A PR 能水解TRIF 和MAVS 两种人类免疫蛋白，从而干扰人体对HCV 的先天性免疫应答。 NS3/4A PR 还可通过调控IFN-3 的表达水平，破坏细胞内 免疫通 …

2019年8月1日 · In this proof-of-concept study, the authors use telaprevir, which binds hepatitis C virus (HCV) NS3/4A protease, to target the protease for protein degradation, and show inhibition of wildtype...

3 天之前 · The classification of Hepatitis C virus (HCV) NS3 inhibitors is essential for identifying potential antiviral agents through computational methods. This study aims to develop an optimized machine learning (ML) model using random forest (RF) and molecular fingerprints to accurately classify HCV NS3 inhibitors. A dataset of 965 molecules was retrieved from the ChEMBL database, and 290 bioactive ...