2020年7月1日 · CLDN1- and OCLN-targeting compounds are HTAs efficiently inhibiting HCV entry in host cells. HTAs combination with current DAAs could show promise for treating HCV infection. The hepatitis C virus (HCV) is a major cause of liver diseases ranging from liver inflammation to advanced liver diseases like cirrhosis and hepatocellular carcinoma (HCC).

To decipher the mechanism underlying virus slow-down upon OCLN recruitment, we performed CRISPR knockout (KO) of CLDN1, an HCV entry factor proposed to act upstream of OCLN. Although CLDN1 KO potently inhibits HCV infection, OCLN kept accumulating underneath the particle, indicating that OCLN recruitment is CLDN1 independent.

Our study suggests that the remarkable natural splicing diversity of OCLN might contribute to HCV tissue tropism and possibly modify the outcome of HCV infection in humans. Genetic factors crucial for regulation of OCLN expression and susceptibility …

Occludin是一种膜整合蛋白，在紧密连接（tight junction, TJ）细胞旁通透性屏障的形成和调节中发挥作用。 细胞旁转运（Paracellular transport）是指分子在相邻上皮细胞之间运输，而紧密连接可调节细胞旁路渗透性并维持细胞极性。 Occludin当细胞缺乏紧密连接时，可以诱导粘附。 Occludin可以作为肝细胞中丙型肝炎病毒（HCV）的共同受体。 Occludin在上皮细胞和内皮细胞的紧密连接处表达。 在肾脏中高度表达，在睾丸中未检测到。 Occludin含有多个异构体，不同 …

2010年11月10日 · Recent findings have implicated tight junction (TJ) protein Occludin (OCLN) as an essential factor for hepatitis C virus (HCV) to enter human hepatocytes. To gain insights into OCLN-mediated HCV entry, we created a panel of OCLN deletion mutants and found that without impairing OCLN's cell surface l …

2010年11月10日 · Our study indicate that the EL2 domain plays a pivotal role in OCLN-dependent HCV entry. Ploss et al. (2009) previously demonstrated that OCLN EL2 harbors species-specific determinant for HCV entry. Here, we show that OCLN EL2 is both required to confer HCVpp infectivity and its ability to precipitate with HCV E2.

2008年12月3日 · A tight junction (TJ) protein, claudin-1 (CLDN1), was identified recently as a key factor for hepatitis C virus (HCV) entry. Here, we show that another TJ protein, occludin, is also required for HCV entry.

Through an iterative cDNA library screening approach we have identified human occludin (OCLN) as an essential HCV cell entry factor that is able to render murine cells infectable with HCVpp.

2020年2月3日 · After lateral translocation of virions to tight junctions, claudin-1 (CLDN1) and occludin (OCLN) are essential for entry. Clathrin-mediated endocytosis engulfs HCV particles, which fuse with endosomal membranes after pH drop. Uncoating of the viral RNA genome in the cytoplasm completes the entry process.

2024年5月23日 · Using an iterative cDNA library screening approach, Ploss et al. (2009) showed that human occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. Similarly, occludin is required for the HCV susceptibility of human cells, because its overexpression in uninfectable ...