Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

There are limited numbers of models to study hepatocellular carcinoma (HCC) in vivo in immunocompetent hosts. In an effort to develop a cell line with improved tumorigenicity, we derived a new cell line from Hepa1-6 cells through an in vivo passage in C57BL/6 mice.

2001年11月1日 · Hepatocellular carcinoma is a lethal disease and methods that develop effective cellular-based immunotherapy are needed. We retrovirally transduced non-immunogenic mouse Hepa1-6 hepatoma cells with the gene encoding the membrane form of macrophage colony stimulating factor (mM-CSF).

The wildly used and established HCC cell line, Hepa 1-6, is derived from C57BL/6J mice. Hence, if Hepa 1-6 cell line is transplanted into any mice strain besides C57BL/6J, it cannot be called as a syngeneic mouse model.

The Hepa 1-6 cell line remains a widely used and valuable tool for studying the biology of HCC and testing potential therapies. Its high tumorigenicity and genetic and molecular similarities to human HCC make it a useful model for preclinical studies.

Here we have investigated insulin signaling, gluconeogenic gene expression, glucose production, and fatty acid synthase abundance in primary hepatocytes and in HepG2, Hepa 1–6, and McARH7777 hepatoma cell lines.

The Hepa 1-6 xenograft model enables the study of tumor growth inhibition of therapeutic agents such as expression of hTERTC27 by a recombinant adenovirus or RNA replacement therapy through miRNA regulation (i.e. miR-122 or miR-34).

To cite this cell line use: Hepa 1-6 (RRID:CVCL_0327) Comments Characteristics: Secretes several liver products including albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase (ECACC=92110305).

Hepa 1-6 Model Information: Derived from the BW7756 mouse hepatoma that spontaneously arose in a C57/L mouse, this murine model of hepatocellular carcinoma (HCC) offers unparalleled insights for evaluating the efficacy and immuno-PD changes following anti …

Hepa 1-6细胞是C57/L小鼠中产生的BW7756小鼠肝癌的衍生株。 Q：Hepa 1-6细胞成瘤性怎么样？ A：背景资料中显示该细胞可以成瘤：Hepa 1-6 Cells https://www.cytion.com/Hepa-1-6-Cells/400474。 Q：为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢？ A：部分细胞是会出现多种培养条件的，我们公司优先选择引种来源的培养条件以及建系者所用培养 …