Plasmid HH-gRNA-HDV-Shuttle-Vector from Dr. Marianne Bronner's lab contains the insert HH-gRNAbackbone-HDV and is published in Development. 2021 Mar 9. pii: dev.193565. doi: 10.1242/dev.193565. This plasmid is available through Addgene.

此外，与tRNAGly序列释放引导RNA的过程类似，在引导RNA的5'端和3'端各加上一种核酶序列，5'端是Hammerhead(HH)type ribozyme，3'端是Hepatitis delta virus(HDV)ribozyme，这种结构形成的引导RNA分子称为RGR(Ribozyme-gRNA-Ribozyme，RGR)。

2022年11月30日 · The scgRNA consists of a gRNA flanked by two self-cleaving ribozymes: a hammerhead (HH) ribozyme located 5′ to the gRNA and a hepatitis delta virus (HDV) ribozyme located 3′ to the gRNA.

具体研究结果简述如下： 1、基于课题组之前构建的植物双Pol II型启动子表达系统和HH-sgRNA-HDV表达单元（Tang et al., 2017），构建了植物Cas12b基因组定向编辑核心系统，基于水稻原生质体转化，针对水稻内源检测到有效的基因组定向敲除编辑事件。

2015年12月16日 · Hammerhead (HH) ribozyme 13 and hepatitis delta virus (HDV) ribozyme 14 perform site-specific self-cleavage, resulting in cleavage products with 2′,3′-cyclic phosphate and 5′-hydroxyl...

(Empty Backbone) Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

2021年2月2日 · 在grna的5'端引入锤头型核酶hh和3'端引入丁型肝炎病毒（hdv）核酶将介导转录本自我剪切去掉两端的修饰，避免后续出核而无法行使引导和靶向的功能。

hh-hdv是一种依赖乙型肝炎病毒（HBV）进行复制的缺陷型RNA病毒。 它主要感染已经感染了HBV的个体，并可能加剧乙型肝炎的病情。 当我们将hh-hdv基因导入植物载体时，我们的目的是利用植物的生

2018年9月6日 · In yeast 17, mammalian cells 15, 18, and zebrafish 19, hammerhead (HH) and hepatitis delta virus (HDV) ribozyme have been used to cleave RNA in vivo to produce multiple sgRNAs from a single...

The self-cleaving RNA sequences, or ribozymes, in the genomic and antigenomic strands of hepatitis delta virus (HDV) RNA fold into structures that are similar to each other but distinct from those of small ribozymes associated with the RNA replicons that infect plants.