2021年10月15日 · In this study, we developed functional oligonucleotides that mimic the function of 7SK (7SK mimics) as novel inhibitors of HIV replication. We defined the essential region of 7SK regarding its suppressive effects on transcriptional downregulation using an antisense strategy.

2018年10月15日 · Nature Communications - The HIV Tat protein recruits a host elongation factor from the cellular 7SK complex to the viral TAR RNA to ensure transcriptional elongation. Here, Pham et al. solve...

HIV requires P-TEFb for its replication 12, 15 and this suggests that the 7SK snRNP could be targeted for silencing and/or re-activating viral gene expression in the infected host. Other diseases that are impacted by P-TEFb include cardiac hypertrophy, steroid-dependent or responsive cancers, leukemias, lymphomas, as well as inflammatory and ...

Studies have shown that 7SK is a key regulator of P-TEFb dynamic homeostasis and activity, and P-TEFb is a specific host cofactor of HIV-1 transcription . P-TEFb is part of the multi-subunit hyperelongation complex (SEC), which is recruited to viral LTR by HIV-1-encoded Tat protein to stimulate viral elongation [ 160 , 161 ].

2014年5月2日 · In this study, we demonstrate that the 7SK small nuclear ribonucleoprotein (snRNP) complex is recruited to the HIV-1 promoter via newly-synthesized HIV-1 nascent transcripts (short transcripts) in an hnRNP A1-dependent manner and negatively regulates viral transcript elongation.

Now D'Orso and Frankel 1 report the unexpected findings that Tat assembles with the inhibitory 7SK small nuclear ribonucleoprotein (snRNP) harboring the catalytically inactive form of P-TEFb and...

The 7SK snRNA sequesters P-TEFb, a general transcription elongation factor and human co-factor for HIV-1 Tat protein, into the catalytically inactive 7SK snRNP Little is known about how 7SK RNA is regulated to perform this function. Here, we show that most of 7SK is pseudouridylated at position U250 …

2017年10月1日 · Our findings identify a novel role for HIV-1 TAR in the absence of Tat, involving the interference with host cellular immune responses by targeting 7SK RNA -mediated gene expression and P-TEFb inactivation. HIV-1 latency is established by an interplay of different mechanisms, which render the HIV provirus transcriptionally silent.

2021年1月1日 · The expression of short hairpin RNAs (shRNAs) in cells has many potential therapeutic applications, including as a functional cure for HIV. The RNA polymerase III promoters H1, 7SK, and U6 have all been used to express shRNAs. However, there have been no direct and simultaneous comparisons of shRNA …

2016年8月24日 · Our present data support the idea that the 7SK RNP plays an inhibitory role in HIV's transcription and escape from latency. The demonstration that this RNP can be destabilized by reducing 7SK RNA pseudouridylation, which activates HIV transcription and promotes latency reversal, implicates the DKC1–box H/ACA RNPs as a promising new target for ...