2022年12月1日 · A CRISPR/Cas9 introduces double stranded breaks in the HIV-1 LTR and/or viral genes thereby inactivating the proviral genome. Use of multiple gRNAs results in excision or hypermutation of the target sites.

CRISPR-Cas editing platforms (blue scissors) against HIV can be classified into three categories: the single targeting of HIV genes, the dual or multiplex targeting of HIV genes, and a combined targeting of HIV genes and host DNA to boost the activity restriction factors.

CRISPR/Cas9 can be engineered to successfully modulate an array of disease-causing genetic elements. We discuss the diverse roles that CRISPR/Cas9 may play in targeting HIV and eradicating infection. The Cas9 nuclease coupled with one or more small guide RNAs (sgRNAs) can target the provirus to mediate excision of the integrated viral genome.

2018年5月17日 · CRISPR/Cas9 provides a promising new method to target the regulatory genes of HIV-1. In the present study, we constructed a lentiviral vector-based CRISPR/Cas9 system harboring gRNAs with the...

2016年3月4日 · We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently...

Kaminski et al. modulated the CRISPR/Cas9 system by placing the Cas9 gene under the control of a minimal HIV-1 promotor, which is activated by viral transcriptional activator, Tat. This strategy makes Cas9 express in HIV-1 infectious cells and reduces the complications that caused by unnecessary high expression of Cas9 in cells (Kaminski et al ...

2015年3月10日 · We show that engineered human-induced pluripotent stem cells stably expressing HIV-targeted CRISPR/Cas9 can be efficiently differentiated into HIV reservoir cell types and maintain their...

2019年3月22日 · This versatile gene editing technology has been successfully applied to HIV-1/AIDS prevention and reduction in human cells and animal models. Here, we update the rapid progress of CRISPR/Cas9-based HIV-1/AIDS therapy research in recent years and discuss the limitations and future perspectives of its application.

The efficacy of the anti-HIV CRISPR/Cas9 strategy is highly dependent on the gRNA sequence, yet some mutant viral strains show poor or no cleavage at all. Higher CRISPR/Cas9 pressure could delay but not eliminate viral replication when using a combination of distinct gRNAs targeting distinct HIV proviral genes.

2022年2月1日 · Berthoux et al. used a transfection-based CRISPR/Cas9 genome editing approach to successfully mutate TRIM5α into its potentially HIV-1-restrictive version via HDR. This strategy emphasized the importance of biallelic modification of TRIM5α to achieve significant HIV-1 replication inhibition.