2013年7月18日 · Genes encoding HLA class I α-chain have a characteristic structure in which different domains of the protein are encoded by different exons. The leader peptide is encoded by exon 1, and the three extracellular domains (α1, α2, …

2024年12月25日 · Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described.

The alignment tool provides access to pre-compiled alignments for individual HLA genes and sequence features (e.g. specific exons and introns), or alignments of commonly sequenced regions (e.g. exons 2+3).

2009年1月9日 · The human leukocyte antigen (HLA) super-locus is a genomic region in the chromosomal position 6p21 that encodes the six classical transplantation HLA genes and at least 132...

5 天之前 · Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

Full-resolution and exon HLA typing using SpecHLA. With Exome data like WES or RNASeq, only support exon typing. For efficient HLA typing, we strongly recommend utilizing only HLA-related reads.

2024年12月25日 · Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

By choosing a series of primers assessing polymorphism at the relevant regions within exons 2–3 and exon 2 of the Class I and II genes, an HLA typing can be accomplished. SSP-PCR requires about 100 simultaneously performed PCR assays per patient to identify HLA-A, -B, and -DRB allele groups at serological equivalent resolution. More reactions ...

2011年1月18日 · Six HLA Class I exonic regions were amplified by PCR: exons 2 and 3 in each of HLA-A, -B, and -C. Primers were designed to fall within the intronic regions surrounding the exon of interest (Figure (Figure1, 1, Additional File 11). Each primer pair was designed to uniquely target a specific HLA locus without promiscuity.

2024年10月2日 · We developed major histocompatibility complex loss of heterozygosity (LOH), allele-specific mutation and measurement of expression and repression (MHC Hammer). We identified extensive variability...