人类白细胞抗原分子（HLA）是人体主要组织兼容性基因（MHC）,介导实体器官移植中的不同排斥反应。 在器官或骨髓移植过程中，供体与受体的HLA兼容程

Human HLA-A qPCR Primer Pair，即人HLA-A qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

ScienCell 使用我们的 GeneQuery™ HLA PCR+Sanger SBT 分型试剂盒（Cat #GK891/GK892/GK893/GK894/GK895/GK896）提供 HLA 基因分型服务。 只需将您的细胞样本或基因组 DNA 样本寄给我们，我们就会及时提供结果。

2024年10月16日 · HLA typing can be performed using numerous methods, including endpoint PCR, qPCR, Sanger sequencing, and next-generation sequencing (NGS). HLA typing has grown more accessible as NGS...

人类白细胞抗原（human leukocyte antigen，简称HLA），位于 6号染色体 的短臂上（6p21.3），由360万个碱基对组成，包括一系列紧密连锁的基因座，部分基因编码细胞表面抗原，成为每个人的细胞不可混淆的“特征”，是免疫系统区分自身和异体物质的基础。

试剂盒采用实时荧光PCR法，其结合Taqman探针技术和扩增阻碍突变系统设计特异性引物与探针，对HPA和HLA-A、B基因进行定性分析检测。 当探针完整时，不

We present a real-time PCR approach for the identification and subtyping of HLA-DR4 alleles. The technique, which uses sequence-specific primers and probes in conjunction with real-time PCR for the detection and differentiation of target alleles, is rapid, involves minimal hands-on time, and is inexpensive compared to existing methods.

This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11) .

2024年6月18日 · In this study, we developed and validated an in-house quantitative PCR (qPCR) method for the simultaneous detection of HLA-B75 serotype markers (HLA-B*15:02, HLA-B*15:11, and HLA-B*15:21) and validated it using two sets of samples: HLA-typed samples and HLA-screened samples.