2021年4月14日 · HP1a (hereafter termed as HP1, encoded by Su (var)2-5) was discovered in Drosophila 15 and is essential for early embryonic development, as is the mammalian protein HP1β 16, 17. HP1 localizes...

Non-histone chromatin proteins such as the Heterochromatin Protein 1 (HP1) proteins are critical regulators of transcription, contributing to gene regulation through a variety of molecular mechanisms. HP1 proteins are highly conserved, and many eukaryotic genomes contain multiple HP1 …

近日德国, Luca Giorgetti3 和Nicola Iovino课题组在nature上发表了题为HP1 drives de novo 3D genome reorganization in early Drosophila embryos 的研究结果。 他们通过结合染色体构象捕获（Hi-C）、 染色质免疫沉淀 和高通量测序（ChIP–seq）、3D-DNA 荧光原位杂交 （3D-DNA-FISH）和聚合物模拟，发现异染色质蛋白1a（HP1a）在果蝇早期发育过程中对从头建立三维基因组是必不可少的。 他们发现HP1a的结合丝粒周围异染色质是建立着丝粒周围区域所必需的。 …

2024年5月24日 · Here, we examine the roles of HP1a and methylated histone H3 lysine 9 (H3K9), two hallmarks of constitutive heterochromatin, in determining 3D genome organization during Drosophila melanogaster development.

2021年7月14日 · Drosophila Heterochromatin Protein 1a (HP1a) is essential for heterochromatin formation and is involved in transcriptional silencing. However, certain loci require HP1a to be transcribed. One model posits that HP1a acts as a transcriptional silencer within euchromatin while acting as an activator within heterochromatin.

2014年7月1日 · ChIP–chip (chromatin immunoprecipitation [ChIP] coupled with microarray analysis) and ChIP-seq (ChIP combined with deep sequencing) experiments demonstrate that HP1a is indeed highly enriched over pericentric heterochromatin but in addition binds active genes in the euchromatin and heterochromatin (Piacentini et al. 2003; de Wit et al. 2007 ...

Here, by combining chromosome conformation capture (Hi-C), chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), 3D DNA fluorescence in situ hybridization (3D DNA FISH) and polymer simulations, we show that heterochromatin protein 1a (HP1a) is essential for de novo 3D genome organization during Drosophila early development.

2018年5月4日 · Our results suggest that HP1a is required for piRNA biogenesis predominantly from the regions close to centromeres and telomeres. We also show that HP1a functions upstream to piRNA processing,...

To identify the borders of pericentric heterochromatin domains more precisely, we analyzed publicly available ChIP-seq profiles in S2 cells for HP1a, along with Su (var) 3-9, Su (var) 3-7, and H3K9me3 (Figure 5, see also Material and Methods). We examined a section near the chromocenter (the black circle at the top of thde schematic ...

2022年1月18日 · Here, we report a previously uncharacterized function of 53BP1 at heterochromatin, where it undergoes liquid-liquid phase separation (LLPS) with the heterochromatin protein HP1α in a mutually...