Phase separation drives heterochromatin domain formation
2017年6月21日 · We show that Drosophila HP1a protein undergoes liquid–liquid demixing in vitro, and nucleates into foci that display liquid properties during the first stages of heterochromatin …
Phosphorylated HP1α–Nucleosome Interactions in Phase …
2023年10月23日 · Our experiments indicate that phosphorylated human HP1α does not cause any major rearrangements to the nucleosome core, in contrast to the yeast homologue Swi6. …
Epigenetic marks uniquely tune the material properties of HP1α ...
2024年6月4日 · FRAP measurements with ∼1% Alexa488-labeled HP1α revealed that HP1α molecules remain dynamic within HP1α-DNA condensates for all three DNA lengths, with …
Multivalency governs HP1α association dynamics with the silent ...
2015年6月18日 · Here we elucidate the mechanism how heterochromatin protein 1α (HP1α), a multivalent effector, is efficiently recruited to the silent chromatin state (marked by …
FRAP calibrated polymer model of chromatin with HP1a predicts ...
We develop a simple model containing spherical particles to mimic HP1a protein and a single-chain polymer representing the chromatin. We account for HP1a self-interaction and its …
HP1α targets the chromosomal passenger complex for activation …
2018年2月21日 · Whether and how heterochromatin protein 1α (HP 1α) influences CPC localisation and function during mitotic entry is less clear. Here, we alter HP 1α dynamics by …
Distinct characteristics of HP1α and DNA in condensates. A. FRAP …
Furthermore, FRAP experiments of HP1α-DNA condensates labeled with YOYO-1 exhibit recovery rates proportional to DNA length: the longer the DNA, the slower the rate of recovery …
2018年2月21日 · The single amino-acid substitutions I165E or W174A within the CSD completely elimi-nated the mitotic delay caused by HP1 tethering (Fig 1G). Impor- W174A tantly, in FRAP …
FRAP analysis of GFP-HP1 shows that HP1 proteins are
Loss of heterochromatin proteins HP1α and HP1β causes deformation of PCH, with reduced H3K9 trimethylation (H3K9me3) and HP1γ levels, absence of H4K20me3 and upregulated …
HP1 proteins compact DNA into mechanically and positionally …
In mammals, HP1-mediated heterochromatin forms positionally and mechanically stable genomic domains even though the component HP1 paralogs, HP1α, HP1β, and HP1γ, display rapid on …