HP1a Inhibits Sox2 (A, C, E, G, I, K, M, and Q) Embryos electroporated with BrmK755R (A), GFP (C), or HP1a (E) in the neural plate, DHP1a (G) or GFP (I) in the nonneural ectoderm, and DHP1a (K), GFP (M), and DHP1a together with BrmK755R (Q) in the extra-embryonic epiblast and stained for Sox2 (purple).

2021年6月9日 · Heterochromatic histone methylation controls nucleus and chromosome stiffness, but the contribution of heterochromatin protein HP1α (CBX5) is unknown. We used a novel HP1α auxin-inducible degron human cell line to rapidly degrade HP1α.

In this study, we identified several chromatin modulators and RNA binding proteins interacting with Sox2 in different nuclear fractions of embryonic stem (ES) cells, by Stable Isotope Labelling by Aminoacids in Cell culture (SILAC) technology (Ong et al., 2002), coupled with immunoprecipitation and mass spectrometry-based quantitative proteomics.

2023年10月23日 · Our experiments indicate that phosphorylated human HP1α does not cause any major rearrangements to the nucleosome core, in contrast to the yeast homologue Swi6. Instead, HP1α interacts specifically with the methylated H3 …

2018年5月3日 · 为了开展这一研究，清华大学颉伟组优化了现有的细胞染色质开放区域定位技术(简称为miniATAC-seq，Assay for Transposase Accessible Chromatin with high-throughput sequencing)，实现了在极少量细胞（最低可只用到20个细胞，而未优化前在小鼠中的实验需要上百个细胞）水平上进行开放染色质区域的检测。 进而与郑州大学第一附属医院孙莹璞研究组紧密合作，揭示了人类早期胚胎发育过程中开放染色质的调控规律。 最后通过与清华大学医学院那 …

2016年1月26日 · In this study, we conducted a comprehensive study of SOX2 in hESCs and their progenies along neural differentiation. We compared SOX2 global binding profiles, SOX2-regulated genes and SOX2...

Heterochromatic histone methylation controls nucleus and chromosome stiffness, but the contribution of heterochromatin protein HP1α (CBX5) is unknown. We used a novel HP1α auxin-inducible degron human cell line to rapidly degrade HP1α.

2020年4月22日 · Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally...

2022年12月20日 · 异染色质蛋白 1α (HP1α) 是染色质组织的重要组成部分。 有人提出 HP1α 通过液-液相分离 (LLPS) 发挥作用，这使其能够将染色质压缩到转录抑制的异染色质区域。 在体外，HP1α 可以在其 N 末端延伸 (NTE) 磷酸化和/或通过与 DNA 和染色质相互作用时发生相分离。 在这里，我们结合计算和实验方法来阐明驱动这些过程的分子相互作用。 在磷酸化驱动的 LLPS 中，HP1α 可以交换二聚体内部铰链-NTE 与二聚体间接触的相互作用，这也导致从压缩到扩展的 …

2024年10月18日 · HP1a increases the liquidity of chromatin condensates while dramatically differentiating the viscoelastic properties of Me- chromatin versus U-chromatin. Mutating key residues in HP1a show that HP1a interactions with itself and chromatin determine the relative interfacial tension between chromatin compartments, however the formation of ...