Hemoglobin (Hb) A2' is a hematologically silent variant of HbA2 that is detected easily by high-performance liquid chromatography (HPLC), where it elutes in the S window. Our purposes were to define diagnostic criteria for the HbA2' trait using the Variant II …

Several studies showed an excellent correlation between capillary electrophoresis and high performance cation-exchange chromatography (HPLC) for the qualitative and quantitative haemoglobin analysis (2 - 4). The most used methods for HbA 2 measurement are CE, HPLC and less frequently the microchromatography on DE-52 (5 - 8).

The present findings show HPLC as an excellent, powerful diagnostic tool for the direct identification of haemoglobin variants with a high degree of precision in the quantification of normal and abnormal haemoglobin fractions. CE-HPLC (β-thal short program) may be a valuable tool in rapid diagnosis of a varied spectrum of haemoglobinopathies.

The performance characteristics of both machines were compared for the detection of the 5 most common hemoglobin variants: Hb A, HbF, HbS, Hb C, and HbE. On comparing the HbA2 values, the HPLC showed higher values for HbA2 as compared to HbCZE, while the HbF and HbS measurement agreement was good between both methods.

2011年4月5日 · High performance liquid chromatography (HPLC) identifies it as a small S-window peak with a mean retention time of 4.59 ± 0.03 minutes. This study aims at describing the frequency of detection of HbA2' by HPLC in Pakistan and its confirmation at a molecular level.

HPLC was compared with column anion exchange chromatography for HbA2 measurement, and radial immunodiffusion, or alkaline denaturation for HbF measurement. The reliability of HbA2 measurement by HPLC for the detection of beta thalassaemia and HbE was assessed in 200 consecutive samples for routine thalassaemia screening.

High-performance liquid chromatography (HPLC) is a technique introduced for the accurate diagnosis of hemoglobinopathies and thalassemias. The advantage of the HPLC system is the excellent resolution, reproducibility & quantification of several normal & abnormal hemoglobin resulting in accur …

Hb A2 is eluted from the column resin by a programmed buffer gradient of increasing ionic strength. Hb A2 measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel and red cell indices and iron supply measurements. HbA2, Hb A2.

The reliability of HbA2 measurement by HPLC for the detection of beta thalassaemia and HbE was assessed in 200 consecutive samples for routine thalassaemia screening. Results: HPLC was rapid, technically easy, and gave good precision and reproducibility.

在这项研究中，我们尝试使用两种方法（HPLC 和 HbCZE）比较两年内医生指示的血红蛋白病筛查送出的 536 份全血样本中的 HbA2 值。 这包括产前妇女、对铁无反应的贫血患者，以及儿童或兄弟姐妹被诊断患有血红蛋白病或地中海贫血的家庭筛查病例。 比较了两台机器的性能特征，以检测 5 种最常见的血红蛋白变体：Hb A、HbF、HbS、Hb C 和 HbE。 在比较 HbA2 值时，HPLC 显示 HbA2 值高于 HbCZE，而两种方法之间的 HbF 和 HbS 测量一致性良好。 HbA2 的正常范 …