MMEJ在M期和S早期处于激活状态，HDR处于非激活状态，NHEJ、HDR和MMEJ修复的平衡因机体而异。 MMEJ并不像HDR那样产生"完美修复"，但它比NHEJ更具有可预测性。

2020年4月3日 · 对于DSBs损伤，主要有 非同源末端链接（Non-homologous end joining，NHEJ） 和 同源重组 (Homologous recombination，HR） 两种，单链退火修复一般发生在串联同源DNA序列中，研究较少 [1]（图1）。

Microhomology-mediated end joining (MMEJ), also known as alternative nonhomologous end-joining (Alt-NHEJ) is one of the pathways for repairing double-strand breaks in DNA.

2017年5月19日 · Here we devise a homology-mediated end joining (HMEJ)-based strategy, using CRISPR/Cas9-mediated cleavage of both transgene donor vector that contains guide RNA target sites and ∼ 800 bp of...

2017年1月13日 · MMEJ is a mutagenic DSB repair mechanism, which always associates with deletions flanking the break sites and contributes to chromosome translocations and rearrangements. Recent study indicated that MMEJ is used with appreciable frequency even when HR is available [13].

2017年6月5日 · 该研究设计了一种以同源臂介导的末端接合（Homology-Mediated End Joining, HMEJ）为基础的基因敲入策略，它在很多种系统（体外培养的细胞、动物胚胎和体内组织）中比现有的基因敲入策略的效率都要高。 基于更高效的编辑效率和更好的精确度，HMEJ介导的基因敲入方法为一系列应用，包括基因编辑动物模型的建立、疾病的靶向基因治疗等提供了非常大的应用前景。 在体外使用基于HMEJ的方法进行基因组编辑.

2019年4月4日 · NHEJ通路是大多数细胞中占主导地位的DSB修复通路。 MMEJ通路通过末端切除来发现断裂两侧的微小同源序列，这些同源序列可用于模板化断裂端融合。 PARP-1可通过MMEJ通路调控DSB修复。 用PARP-1抑制剂rucaparib治疗细胞可降低DSB在MMEJ修复通路中的 …

2013年4月22日 · Using a MMEJ and HR competition repair substrate, we demonstrated that MMEJ with short end resection is used in mammalian cells at the level of 10–20% of HR when both HR and nonhomologous end joining are available. Furthermore, MMEJ is used to repair DSBs generated at collapsed replication forks.

Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism that involves alignment of microhomologous sequences internal to the broken ends before joining, and is associated with deletions and insertions that mark the original break site, as …

2019年4月4日 · Gaps: The NHEJ-mediated homology-independent targeted integration (HITI) system is especially useful for in vivo gene knock-in with high efficiency. however, this system cannot assign multiple donors with different genomic loci simultaneously because there are no homology arms on the targeting donor vector for the HITI system.