Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen ...
2023年8月17日 · In the present work, the effect of H 2 O 2 on the inactivation of horseradish peroxidase (HRP) was evaluated. Three states of HRP (E 0, E 2, and E 3) were identified. While in the absence of H...
UV-vis absorption spectrum of HRP (0.58 mg = mL, solid line) …
In the direct-competitive enzyme-linked immunosorbent assay (dc-ELISA) approach, an ochratoxin A-horseradish peroxidase (OTA-HRP) synthesized conjugate was utilized. Fluorescence studies revealed...
Activity and stability of horseradish peroxidase in hydrophilic room ...
2007年6月1日 · Horseradish peroxidase (HRP) is one of the most interesting heme proteins studied for the electrocatalytic reduction of H 2 O 2. The investigation is informative for understanding the kinetics and thermodynamics of biological redox processes in living systems.
UV-Vis detection of hydrogen peroxide using horseradish peroxidase ...
2020年10月1日 · Here in this work, HRP/copper phosphate (Cu 3 (PO 4) 2) hybrid nanoflowers (HRP-HNFs) were prepared for ultraviolet-visible (UV–vis) detection of H 2 O 2. HRP-HNFs showed wide linear range (100 nM to 100 μM) and good selectivity toward H 2 O 2 detection, moreover, HRP-HNFs have good reusability and long-term storage stability, which suggests ...
UV- Vis absorption spectra of ( a ) HRP solution (0.6 μ M) and
We report on a novel matrix of solgel organic–inorganic nanocomposite that was fabricated from silica sol gel and dextran. It was used for the immobilization of horseradish peroxidase (HRP) to...
基于2,6-二- (2-吡啶基)-4-吡啶甲酸的紫外法检测辣根过氧化物酶浓度
本文以UV-Vis技术测定HRP催化H 2 O 2 氧化配体L的体系。 结果表明该体系中的HRP酶促反应灵敏度高，反应迅速，重现性高且稳定。 本方法可用于游离HRP的检测，操作简便，有望用于医药工业、食品检测、环境监测以及探究生命体信息规律等诸多领域，可为HRP检测的发展和完善提供有用信息和多样性选择。 2. 实验部分. 2.1. 实验试剂与仪器. 2,6-二- (2-吡啶基)-4-吡啶甲酸，辣根过氧化物酶 (HRP)，阿拉丁；30% H 2 O 2，NaOH，KOH，NaHSO 3，无水乙醇，分析纯，西 …
UV–Visible absorbance spectra of HRP (a) and HRP-MWNTs (b) film
We develop a novel strategy for the biosensing application of hydrogen peroxide (H2O2) using nanoscaled Au–horseradish peroxidase (HRP) composite thin film synthesized by a liquid–liquid...
紫外光照下辣根过氧化物酶酶活变化及机制 - 豆丁网
2015年10月29日 · 摘要：实验发现不同波长光对辣根过氧化物酶（HRP）酶活的钝化程度不同，我们采用紫外、荧光及圆二色（CD） 等方法对光还原导致酶活变化及其机制进行研究。
Dual-color plasmonic enzyme-linked immunosorbent assay based …
2016年9月7日 · In the presence of targets, HRP catalyzes the oxidation of TMB (colorless) into TMB 2+ (yellow) 6, and the concentration of targets is quantified on the basis of the optical density of the yellow...
Horseradish peroxidase compound I as a tool to investigate …
2011年5月1日 · Previously, we showed that the characteristic UV–Vis spectrum of horseradish peroxidase compound I, produced from the oxidation of horseradish peroxidase by hydrogen peroxide, is a simple, reliable, and useful tool to determine the second-order rate constant of the reaction of reactive protein-Cys with hydrogen peroxide and peroxynitrite.