一维氢谱和碳谱描述了单个原子信息，2D NMR中的1H-1H COSY和HSQC，组合了相近原子间的信息，通过以上方式，我们仅知道分子的片段信息，只有再把片段与片段信息组合起来，我们才可知道整个分子的信息。 HMBC谱可联系相隔2~3个共价键的氢核和碳核，如-CHa-CHb-，Ha的谱峰位置可对应Hb的碳。 这里，相隔2个共价键的氢核-碳核点信号通常比相隔3个共价键的氢核-碳核响应会强些。 像-CHa-CO-CHb-片段，尽管中间被羰基C=O隔断，通过HMBC谱，Ha与Hb上的 …

The 2D HSQC (Heteronuclear Single-Quantum Correlation) experiment permits to obtain a 2D heteronuclear chemical shift correlation map between directly-bonded 1H and X-heteronuclei (commonly, 13C and 15N) .

1H-15N HSQC原理介绍：1H-15N HSQC谱，全称为1H-15N 异核单量子相干谱（1H-15N Heteronuclear Single Quantum Coherence spectroscopy），是生物核磁研究中最基础也是最常用的一个二维谱。图四所示是1H-15N HSQC核磁脉冲序列：

The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR.

2023年2月11日 · Hetereonuclear Single Quantum Coherence (HSQC) is used to determine the proton to carbon or heteroatom (often nitrogen) single bond correlations. It is also known as heteronuclear multiple quantum coherence (HMQC).

The 2D HSQC-NOESY experiment is an hybrid inverse experiment consisting of an initial HSQC pulse train followed by a NOESY building block. In the first step, magnetization is transferred from the X nucleus (13C or 15N) to the directly-bonded proton nuclei via 1 J(CH).

2010年7月2日 · 2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example 1 H, 15 N-HSQC correlates chemical shifts within NH groups. For similar correlations over multiple bonds 2D HMBC experiment can be used.

2023年12月25日 · 多糖的结构解析中，HSQC二维核磁是开展糖苷键连接确认的基础谱图。 从有机物的HSQC解谱而言，代表的相连接的C-H相关，比如乙醇CH3-CH2-OH，其HSQC谱图中，甲基的质子和甲基碳有相交叉的信号，而亚甲基质子和亚甲基…

二维 (2d) 1 h– 13 c 异核单量子相干 (hsqc) 越来越多地应用于代谢组学研究，因为与一维 (1d) 1 h nmr 相比，它可以大大提高分辨能力。然而，用于基于 2d nmr 的代谢组学的峰匹配和对齐工具等预处理方法落后于 1d 1的类似方法基于 h nmr 的代谢组学。跨多个样本正确匹配和对齐 2d nmr 光谱特征对于后续的多 ...

The ge-2D HSQC experiment is the gradient-enhanced version of the conventional 2D HSQC experiment in which coherence selection is achieved by means of PFG. Thus, clean 2D HSQC spectra can be recorded in a single scan per t 1 increment without need for phase cycle when sample concentration is high.