The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR. The experiment was first described by Geoffrey Bodenhausen and D. J. Ruben in 1980. [1]

异核单量子关系（HSQC）, 隶属于NMR（核磁共振）C-H COSY 谱图 中的一种，此外还有多键 碳氢 关系谱 HMBC 及异核多量子关系 HMQC。 HSQC给出的信息是直接相连的 碳氢 关系，给出一键CH连接问题，而不能解决碳与季碳相连的问题，或隔碳相连的问题。 HMQC 和HSQC,这两种图谱的作用与H-CCOSY相当，由于是反向实验，灵敏度高。 二者相比HSQC优点更多一些。 当 样品量 较多时，宜做H-CCOSY。 异核单量子关系（HSQC）, 隶属于NMR（核磁共振）C-H …

In this study, we employed a combination of genome mining and heteronuclear single quantum coherence (HSQC)-based small molecule accurate recognition technology (SMART) technology to search for fernane-type triterpenoids. ... 2 Key Laboratory for Enzyme and Enzyme-like Material Engineering of Heilongjiang, College of Life Science, Northeast ...

2023年6月20日 · We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13 C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [ 13 C, 1 H]-HSQC cross-peaks free of one-bond 13 C– 13 C couplings.

2020年6月25日 · We demonstrated that a phase-sensitive HSQC pulse sequence with short scan times enables detection of 2-HG and 6-phosphogluconate/gluconate in live cancer cells as well as tumors ex vivo; this...

2023年5月5日 · In this work, methyl-based 2D [1 H-13 C]-heteronuclear single-quantum correlation (HSQC) NMR, at natural abundance, has been used to profile the enzymatic activity of the commercially available enzyme drug. The [1 H-13 C]-HSQC NMR spectra of the protein reveal the role of a flexible loop segment in the activity of the enzyme, in solution ...

2023年6月20日 · Cell-free protein synthesis using eCells allows production of amino acids from inexpensive 13 C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells.

2023年9月29日 · Herein, we report the backbone resonance assignment for UBE2T without the C-terminal region. The available assignment of the 1 H-15 N-HSQC spectrum will be useful for confirming the hits identified from other methods, mapping ligand binding sites, and understanding the binding modes of UBE2T inhibitors.

2016年10月6日 · Enzymatically generated H 2 S is mainly derived from two pyridoxal-5′-phosphate (PLP)-dependent enzymes responsible for the metabolism of L-cysteine (L-Cys): cystathionine-β-synthase (CBS) and...

Heteronuclear Single Quantum Coherence (HSQC) is a technique used in NMR spectroscopy to simultaneously record 1H and 13C chemical shifts by coupling 1H nuclei with other NMR-active nuclei directly bonded to them, providing structural information in complex mixtures.