The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR.

Heteronuclear Single Quantum Coherence (HSQC) is a technique used in NMR spectroscopy to simultaneously record 1H and 13C chemical shifts by coupling 1H nuclei with other NMR-active nuclei directly bonded to them, providing structural information in complex mixtures.

The HSQC (Heteronuclear Single Quantum Coherence) experiment is used to determine proton-carbon single bond correlations, where the protons lie along the observed F2 (X) axis and the carbons are along the F1 (Y) axis.

Gained Information: The Heteronuclear Single Quantum Correlation (HSQC) NMR experiment is a proton-detected 2D experiment that shows you 1H / 13C one-bond connectivity. You will see your 1H spectrum as your direct F2 axis and your 13C spectrum as your indirect F1 axis.

2023年1月1日 · In this chapter, we highlight the potential of a class of methods called intrinsically quantitative 2D HSQC. They are generated by designing pulse sequences able for each correlation peak to maintain constant or to compensate JHH, JCH, off resonances and T2 effects responsible for the degradation of the quantitativity.

2019年2月25日 · 2D NMR experiments provide chemists with evidence to clarify and confirm resonance assignment. Nowadays every organic chemist uses these experiments called COSY, HMBC and HSQC as routine analytics. Basically, with 2D experiments you correlate some kind of information between two 1D spectra.

Heteronuclear single quantum coherence (HSQC) [41] is extensively used in the analysis of complex mixtures as a means of rapidly and simultaneously recording a range of 1 H and 13 C chemical shifts. The experiment couples, typically, 1 H nuclei with other NMR-active nuclei that are directly bonded to it, and uses magnetisation transfer to ...

COSY experiments provide information about 1H-1H coupling, producing crosspeaks for proton pairs having direct JHH couplings ranging 2.5 to 25 Hz for standard COSY and DQ-COSY, and smaller JHH using (long-range) lr-COSY. TOCSY1 is the last variant of homonuclear 2D experiments that we’ll investigate. The standard COSY is shown below next to TOCSY:

2017年8月30日 · The HSQC signal is a singlet while the HMQC is usually a multiplet as you see certain couplings on it. So generally you should prefer the HSQC over the HMQC. One advantage of the HMQC is that it uses significantly fewer pulses than the HSQC.

HSQC is widely used because it is based on proton detection, which offers much higher sensitivity compared to the conventional 2D HETCOR experiment (also called CH-COSY), which observes the 13C signal. Another popular experiment is the multiplicity-edited HSQC-DEPT experiment, which combines the information you get from an HSQC